REACTIONS AT TERMINI OF TRANSFER-RNA WITH T4 RNA LIGASE
REACTIONS AT TERMINI OF TRANSFER-RNA WITH T4 RNA LIGASE
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DOI：
10.1093/nar/5.10.3665
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发表时间：
1978-01-01
影响因子：
14.9
通讯作者：
UHLENBECK, OC
中科院分区：
文献类型：
--
作者：
BRUCE, AG;UHLENBECK, OC
T4 RNA ligase will catalyze the addition of nucleoside 3′,5′-bisphos-phates Onto the 3′ terminus of tRNA resulting in a tRNA molecule one nucleotide longer with a 3′ terminal phosphate. Under appropriate conditions the reaction is quantitative and, if high specific radioactivity bisphosphates are used, it provides an efficient means forin vitrolabeling of tRNA. Although the 3′ terminal hydroxyl is a good acceptor, the 5′ terminal phosphate in most tRNA is not an effective donor in the RNA ligase reaction. This poor reactivity is due to the secondary structure of the 5′ terminal nucleotide. IfE.colitRNAfMetis used, the 5′ phosphate is reactive and the major product with RNA ligase is the cyclic tRNA.
