Induction of apoptotic cell death leads to the development of bacterial rot caused by Pseudomonas cichorii
Induction of apoptotic cell death leads to the development of bacterial rot caused by Pseudomonas cichorii
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DOI：
10.1094/mpmi-19-0112
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发表时间：
2006-02-01
影响因子：
3.5
通讯作者：
Hikichi, Y
中科院分区：
文献类型：
--
作者：
Kiba, A;Sangawa, Y;Hikichi, Y
菊苣假单胞菌是莴苣细菌性腐烂病的主要病原菌。塌陷和布朗宁的症状，观察生菜叶组织接种后15至24小时（HAI）与P菊苣;超氧阴离子的产生检测在1至6 HAI;和细胞死亡诱导在6 HAI，达到最大值在约9和12 HAI。在3例HAI中还观察到异染色质凝聚和DNA梯状条带。药理学研究表明，诱导细胞死亡和DNA梯状化与从头蛋白质合成、蛋白激酶、细胞内活性氧、DNA酶、丝氨酸蛋白酶和caspase III样蛋白酶密切相关。此外，抑制细胞死亡和DNA梯状化诱导的化学物质也抑制了疾病症状的发展。这些结果表明，凋亡细胞的死亡可能是密切相关的细菌腐烂引起的P菊苣。
Pseudomonas cichorii is the major causal agent of bacterial rot of lettuce. Collapse and browning symptoms were observed in lettuce leaf tissue from 15 to 24 h after inoculation (HAI) with P cichorii; superoxide anion generation was detected at I to 6 HAI; and cell death was induced at 6 HAI, reaching a maximum at approximately 9 and 12 HAI. Heterochromatin condensation and DNA laddering also were observed within 3 HAI. Pharmacological studies showed that induction of cell death and DNA laddering was closely associated with de novo protein synthesis, protein kinase, intracellular reactive oxygen species, DNase, serine protease, and caspase III-like protease. Moreover, chemicals, which inhibited the induction of cell death and DNA laddering, also suppressed the development of disease symptoms. These results suggest that apoptotic cell death might be closely associated with the development of bacterial rot caused by P cichorii.