Molecular characterization of the nucleocapsid protein gene of Newcastle disease virus strains in Japan and development of a restriction enzyme-based rapid identifying method
Molecular characterization of the nucleocapsid protein gene of Newcastle disease virus strains in Japan and development of a restriction enzyme-based rapid identifying method
复制标题
DOI：
10.1007/s00705-004-0309-8
复制
发表时间：
2004-04
影响因子：
2.7
通讯作者：
H. M. Pham;Kyung-Soo Chang;M. Mase;K. Ohashi;M. Onuma
中科院分区：
文献类型：
--
作者：
H. M. Pham;Kyung-Soo Chang;M. Mase;K. Ohashi;M. Onuma
对1930 ~ 2001年主要在日本分离的新城疫病毒（NDV）株的核衣壳蛋白（NP）基因进行了遗传学分析。通过推导的氨基酸序列比较，发现NP蛋白的N-端区域（从1到401个残基）是高度保守的，而C-端区域是高度变异的NDV分离物。基于NP全基因核苷酸序列构建的系统发育树显示，旧（20世纪70年代之前）和新（20世纪80年代之后）分离株可分为两个主要的不同组，即，一组由强毒株组成，另一组由无毒毒株组成。通过使用Pst I的限制性酶切分析，没有强毒株被切割，而无毒毒株被切割。本研究结果可作为鉴别NDV分离株的简易初筛试验。
Nucleocapsid protein (NP) gene ofNewcastle disease virus(NDV) strains mainly isolated in Japan from 1930 to 2001 was genetically characterized. By deduced amino acid sequence comparison, the N-terminal region (from 1 to 401 residues) of the NP protein was found to be highly conserved, while the C-terminal region was highly variable among the NDV isolates. A phylogenetic tree construct based on the nucleotide sequence of the complete NP gene revealed that the old (prior to 1970s) and the new (after 1980s) isolates could be classified into two major different groups, i.e., a group comprising virulent strains, and another group composed of avirulent strains. By restriction enzyme analysis usingPstI, none of the virulent strains were cleaved, while avirulent strains were cleaved. The results may be useful for simple primary screening test for differentiating NDV isolates.