Proteomics Profiling of Single Organs from Individual Adult Zebrafish
Proteomics Profiling of Single Organs from Individual Adult Zebrafish
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DOI：
10.1089/zeb.2009.0644
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发表时间：
2010-06-01
期刊：
影响因子：
2
通讯作者：
Zetterberg, Henrik
中科院分区：
文献类型：
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作者：
Abramsson, Alexandra;Westman-Brinkmalm, Ann;Zetterberg, Henrik
模式生物斑马鱼（Danio rerio）被广泛用于发育生物学研究，但也被调查的背景下，越来越多的人类年龄相关疾病。为了促进这些研究，我们在这里提出的蛋白质表达模式的成年斑马鱼器官，包括血液，脑，鳍，心脏，肠，肝脏和骨骼肌。从两条斑马鱼的不同器官中制备蛋白质提取物，并使用液相色谱-高分辨率串联质谱法进行分析。斑马鱼组织在最小分级分离后直接消化并清理（鸟枪法）。使用Mascot软件鉴定蛋白质。总共鉴定了1394种蛋白质，其中644种是非冗余的。其中，373个表现出器官特异性表达模式，57个以前未在蛋白质水平上显示。这些数据强调了在蛋白质水平上增加研究的必要性，以便于选择候选蛋白质进行靶向定量，并完善脊椎动物发育，生物学和疾病的系统遗传网络分析。
The model organism zebrafish (Danio rerio) is extensively utilized in studies of developmental biology but is also being investigated in the context of a growing list of human age-related diseases. To facilitate such studies, we here present protein expression patterns of adult zebrafish organs, including blood, brain, fin, heart, intestine, liver, and skeletal muscle. Protein extracts were prepared from the different organs of two zebrafish and analyzed using liquid chromatography coupled to high-resolution tandem mass spectrometry. Zebrafish tissue was digested directly after minimal fractionation and cleaned up (the shotgun approach). Proteins were identified using Mascot software. In total, 1394 proteins were identified of which 644 were nonredundant. Of these, 373 demonstrated an organ-specific expression pattern and 57 had not been shown on protein level before. These data emphasize the need for increased research at the protein level to facilitate the selection of candidate proteins for targeted quantification and to refine systematic genetic network analysis in vertebrate development, biology, and disease.