Evaluation of estrogen in endometriosis patients: Regulation of GATA-3 in endometrial cells and effects on Th2 cytokines
Evaluation of estrogen in endometriosis patients: Regulation of GATA-3 in endometrial cells and effects on Th2 cytokines
复制标题
子宫内膜异位症患者雌激素的评价：子宫内膜细胞中GATA-3的调节及对Th2细胞因子的影响
DOI：
10.1111/jog.12957
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发表时间：
2016-06-01
影响因子：
1.6
通讯作者：
Liang, Yan-Ming
中科院分区：
文献类型：
--
作者：
Chen, Peng;Wang, Dan-Bo;Liang, Yan-Ming
目的：子宫内膜异位症（Endometriosis，EM）是一种子宫内膜依赖性慢性炎症性疾病，常伴有局部雌激素水平升高和细胞因子水平异常，这些细胞因子受淋巴细胞中加塔-3的调节。本研究旨在探讨雌激素对加塔-3表达的影响以及加塔-3与细胞因子反应的关系。采用免疫组化、定量聚合酶链反应、Western blot、细胞转染、雌激素处理和酶联免疫吸附试验等方法，研究雌激素对EM细胞加塔-3表达的影响及其与EM细胞Th 2免疫状态的关系。加塔-3从细胞质重定位到细胞核。雌激素和加塔-3调节在位子宫内膜细胞中Th 2细胞因子的表达，包括白细胞介素（IL）-6，IL-8和IL-10。结论：加塔-3可能是由雌激素诱导表达的，可能通过调节EM患者在位子宫内膜细胞分泌细胞因子而促进EM的发生和发展。
Aims: Endometriosis (EM) is a hormone-dependent chronic inflammatory disease, usually accompanied by a high level of localized estrogen and abnormal levels of cytokines, which are regulated by GATA-3 in lymphocytes. This study aimed to investigate the role of estrogen on GATA-3 expression and the relationship between GATA-3 and cytokine response.Methods: Endometrial tissues collected from 20 patients who underwent laparoscopic or open surgery were used. Immunohistochemistry, quantitative polymerase chain reaction, Western blot analysis, cell transfection, estrogen treatments and enzyme-linked immunosorbent assays were performed to evaluate the effects of estrogen on GATA-3 expression and the relationship between estrogen-induced GATA-3 and the Th2 immune status of EM.Results: Estrogen regulated the expression of GATA-3 in a dose and time-dependent manner. GATA-3 was relocated from the cytoplasm to the nucleus. Estrogen and GATA-3 regulated Th2 cytokine expression in eutopic endometrial cells, including interleukin (IL)-6, IL-8 and IL-10. Moreover, interferon-gamma and IL-2 were highly expressed in the GATA-3 knockdown groups.Conclusion: In summary, GATA-3 was induced by estrogen and may promote the occurrence and development of EM by regulating the secretion of cytokines in the eutopic endometrial cells of EM patients.