A voltage-activated proton current in human cardiac fibroblasts
A voltage-activated proton current in human cardiac fibroblasts
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DOI：
10.1016/j.bbrc.2005.12.038
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发表时间：
2006-02-10
影响因子：
3.1
通讯作者：
Bois, P
中科院分区：
文献类型：
--
作者：
El Chemaly, A;Guinamard, R;Bois, P
电压激活的质子电流在人类心脏成纤维细胞，使用膜片钳技术的全细胞记录配置测量。据报道。增加洗浴液的pH值会使电流激活阈值向更多的负电位移动，并增加电流振幅和激活速率。将pII梯度改变一个单位，导致电流的反转电位发生51 mV的位移，表明通道携带的质子具有高选择性，即电流。细胞外施加Zn2+可逆抑制电流。激活电流有助于检测细胞内酸中毒条件下的膜电导。我们提出，心脏成纤维细胞中的这种电流在生理条件下调节细胞内pH值和膜电位，以及对缺血等病理条件的反应。(c) 2005爱思唯尔公司版权所有。
A voltage-activated proton current in human cardiac fibroblasts, measured using the whole-cell recording configuration of the patch-clamp technique. is reported. Increasing the pH of the bathing solution shifted the Current activation threshold to more negative potentials and increased both the current amplitude and its rate of activation. Changing the pII gradient by one unit caused a 51 mV shift in the reversal potential of the current, demonstrating a high selectivity for protons of the channel carrying, the current. Extracellularly applied Zn2+ reversibly inhibited the current. Activation of the current contributes to the testing membrane conductance under conditions of intracellular acidosis. It is proposed that this current in cardiac fibroblasts is invoked in the regulation of the intracellular pH and the membrane potential under physiological conditions as well as in response to pathological conditions Such as ischemia. (c) 2005 Elsevier Inc. All rights reserved.