Evaluation of African medicinal plants for their in vitro trypanocidal activity
Evaluation of African medicinal plants for their in vitro trypanocidal activity
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DOI：
10.1016/s0378-8741(96)01463-8
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发表时间：
1996-12-01
影响因子：
5.4
通讯作者：
Brun, R
中科院分区：
文献类型：
--
作者：
Freiburghaus, F;Kaminsky, R;Brun, R
石油醚，二氯甲烷，甲醇和水提取物从24种植物，属于19个家庭，这是在文献中报道的昏睡病（人类非洲锥虫）的传统疗法进行了筛选，在体外活性对布氏锥虫罗得西亚，以及对人类成纤维细胞系（WI-38）的细胞毒性。还评价了天然化合物黄连素和哈满的杀锥虫活性，两者都被证明是杀锥虫的。在13种植物的32种提取物中发现了IC 50值低于10 μ g/ml的有前途的杀锥虫活性。IC 50值低于1 μ g/ml的最具活性的提取物来自于番荔枝、Bussea occurentalis和酸浆。植物提取物显示出适度的选择性指数，与市售的杀锥虫剂相比，其对锥虫具有更明显的选择性毒性。
Petroleum ether, dichloromethane, methanol and water extracts from 24 plants, belonging to 19 families, which are reported in the literature as traditional remedies for sleeping sickness (human African trypanosomiasis) were screened for in vitro activity against Trypanosoma brucei rhodesiense, as well as for cytotoxicity for a human fibroblast cell-line (WI-38). The trypanocidal activity of the natural compounds berberine and harmane, both documented as being trypanocidal, was also evaluated. Promising trypanocidal activity with IC50 values below 10 mu g/ml was found in 32 extracts of 13 plant species. The most active extracts with IC50 values below 1 mu g/ml were derived from Annona senegalensis, Bussea occidentalis and Physalis angulata. The plant extracts showed a modest selectivity index, in contrast to commercially available trypanocides which have a more distinct selective toxicity against trypanosomes.