Gene delivery to tumor cells by cationic polymeric nanovectors coupled to folic acid and the cell-penetrating peptide octaarginine
Gene delivery to tumor cells by cationic polymeric nanovectors coupled to folic acid and the cell-penetrating peptide octaarginine
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通过与叶酸和细胞穿透肽八精氨酸偶联的阳离子聚合物纳米载体将基因递送至肿瘤细胞
DOI：
10.1016/j.biomaterials.2011.06.015
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发表时间：
2011-10-01
期刊：
影响因子：
14
通讯作者：
Tang, Gu-Ping
中科院分区：
文献类型：
--
作者：
Jiang, Qi-Ying;Lai, Li-Hua;Tang, Gu-Ping
将靶配体叶酸（FA）和细胞穿透肽八精氨酸（R8）与由β-环糊精（beta-CyD）和低分子量聚乙烯亚胺（PEI）组成的基因载体（PEI 600-CyD，PC）偶联，形成纳米载体，用于高效基因递送至肿瘤细胞。所得的FA-PC/R8-PC/pDNA三元纳米复合物在体外和体内的叶酸受体（FR）阳性肿瘤细胞中产生了出色的基因转移能力。FR介导的内吞作用和R8介导的跨膜功能一起促成了高转染水平。这项研究提供了一个有前途的手段，以生产基因纳米载体在体内应用。(C)2011爱思唯尔有限公司保留所有权利。
Target ligand folic acid (FA) and cell-penetrating peptide octaarginine (R8) were coupled with the gene vectors (PEI600-CyD, PC) composed of beta-cyclodextrin (beta-CyD) and low-molecular-weight polyethylenimine (PEI, Mw 600) to form nanovectors for highly efficient gene delivery to tumor cells. The resultant ternary nanocomplexes of FA-PC/R8-PC/pDNA produced excellent gene transfaction abilities in the folate receptor (FR)-positive tumor cells in vitro and in vivo. The FR-mediated endocytosis and the R8-mediated transmembrane functionality together contributed to the high transfection levels. This study provides a promising means to produce gene nanovectors for in vivo applications. (C) 2011 Elsevier Ltd. All rights reserved.