Albumin inhibits cytotoxic activity of lysophosphatidylcholine by direct binding
Albumin inhibits cytotoxic activity of lysophosphatidylcholine by direct binding
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DOI：
10.1016/j.prostaglandins.2006.10.006
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发表时间：
2007-02-01
影响因子：
2.9
通讯作者：
Im, Dong-Soon
中科院分区：
文献类型：
--
作者：
Kim, Yu-Lee;Im, Young-Jin;Im, Dong-Soon
发现胎牛血清（FBS）保护Jurkat T细胞免受LPC诱导的细胞毒性。20微摩尔LPC诱导的细胞毒性的80-90%的细胞在培养基中没有FBS的3小时，而50-70%的培养基中有0.5%FBS。然而，在培养基中存在5%FBS的情况下，未观察到LPC诱导的细胞毒性。在检测的溶血磷脂中，LPC具有特异性冷冻毒性，在11种合成LPC中，棕榈酰（C16：0）LPC、硬脂酰（C18：0）LPC和油酰（C18：1）LPC具有显著性冷冻毒性。此外，FBS的细胞保护作用仅在处理前加入时观察到，但在LPC处理后没有观察到，并且在加入细胞前将FBS和LPC预混合可改善LPC诱导的细胞毒性。最后，白蛋白，FBS的主要成分，完全防止LPC诱导的细胞毒性，即使在低至3 μ M的浓度。我们还发现，五个分子的LPC可以顺序结合到一个BSA使用等温滴定量热法。上述结果表明，LPC的细胞毒活性可被血液中的白蛋白减弱。最后，应注意的是，当使用溶解在含有白蛋白的测定缓冲液中的LPC进行实验时，缓冲液中的白蛋白可能影响结果。(c)2006年爱思唯尔公司All rights reserved.
Fetal bovine serum (FBS) was found to protect Jurkat T cells from LPC-induced cytotoxicity. Twenty micromolar LPC-induced cytotoxicity of 80-90% of the cells in media without FBS for 3 h, whereas 50-70% in media with 0.5% FBS. However, LPC-induced cytotoxicity was not observed in the presence of 5% FBS in media. The cyrotoxicity was specific for LPC among lysophospholipids tested and significantly observed with palmitoyl (C16:0) LPC, stearoyl (C18:0) LPC, and oleoyl (C18: 1) LPC among 11 synthetic LPCs. Furthermore, the cytoprotective effect of FBS was observed only when it was added before the treatment, but not after the treatment of LPC, and premixing of FBS and LPC before addition to the cells ameliorated LPC-induced cytotoxicity. Finally, albumin, a major constituent of FBS, prevented completely LPC-induced cytotoxicity even at as low as 3 mu M concentration. We also found that five molecules of LPC could sequentially bind to one BSA using isothermal titration calorimetry. The above results suggest that the cytotoxic activity of LPC could be attenuated by albumin in blood. Finally, it should be cautioned that, when experiments are conducted with LPC dissolved in assay buffers containing albumin, the albumin in the buffer could influence the results. (c) 2006 Elsevier Inc. All rights reserved.