A METHOD FOR DETERMINING CONCENTRATIONS OF RETINOL, TOCOPHEROL, AND 5 CAROTENOIDS IN HUMAN PLASMA AND TISSUE SAMPLES
A METHOD FOR DETERMINING CONCENTRATIONS OF RETINOL, TOCOPHEROL, AND 5 CAROTENOIDS IN HUMAN PLASMA AND TISSUE SAMPLES
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DOI：
10.1093/ajcn/56.2.417
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发表时间：
1992-08-01
影响因子：
7.1
通讯作者：
NANN, SL
中科院分区：
文献类型：
--
作者：
NIERENBERG, DW;NANN, SL
为了探讨血浆和组织中7种脂溶性微量营养素（视黄醇、α-生育酚、叶黄素、隐黄质、番茄红素、α-胡萝卜素和β-胡萝卜素）浓度之间的关系，我们开发了澄清血浆和组织样品并同时定量这7种分析物的方法，采用高效液相色谱法和紫外检测器。通过使用酶消化，然后机械均质化进行组织样品的澄清;避免皂化，并使用生育酚烟酸酯作为内标物。测定了血浆和实体组织小样本的精密度和灵敏度。通过比较使用该方法获得的结果与使用较旧的标准方法获得的结果，评估血浆样品的准确度。结果从应用这种方法对患者的血浆和各种组织样品。这种方法将是感兴趣的研究人员寻求定量这七种微量营养素的固体组织样品，但希望避免在大多数其他报道的技术所需的通常的皂化步骤。
To explore the relation between plasma and tissue concentrations of seven fat-soluble micronutrients (retinol, alpha-tocopherol, lutein, cryptoxanthin, lycopene, alpha-carotene, and beta-carotene), we developed methods for clarifying plasma and tissue samples and quantitating these seven analytes simultaneously by using HPLC with ultraviolet detection. Clarification of tissue samples was performed by using enzymatic digestion followed by mechanical homogenization; saponification was avoided and tocopherol nicotinate was used as an internal standard. Precision and sensitivity for small samples of plasma and solid tissues were determined. Accuracy for plasma samples was assessed by comparing results obtained by using this method with those obtained by using older, standard methods. Results from application of this method to patient samples of plasma and various tissues are presented. This method will be of interest to investigators seeking to quantitate these seven micronutrients in solid tissue samples, but desiring to avoid the usual saponification step required in most other reported techniques.