Characterization of an Escherichia coli mutant which utilizes glycerol in the absence of cyclic adenosine monophosphate.
Characterization of an Escherichia coli mutant which utilizes glycerol in the absence of cyclic adenosine monophosphate.
复制标题
在不存在环单磷酸腺苷的情况下利用甘油的大肠杆菌突变体的表征。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
H. Yamazaki
中科院分区：
文献类型：
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作者：
A. Fraser;H. Yamazaki
甘油的有氧分解代谢依赖于指定甘油激酶的glpK操纵子和指定sn-甘油-3-磷酸（G3P）脱氢酶的glpD操纵子的表达。目前还不清楚这些操纵子的表达是如何依赖于腺苷3',5'-环单磷酸腺苷（cAMP）的。我们已经分离出一个启动子样突变（CA8306B），由于glpK操纵子的突变，它可以在没有cAMP的情况下利用甘油。甘油激酶和G3P脱氢酶在CA8306B及其野生型亲本CA8000中均有诱导作用。CA8306B诱导的甘油激酶水平为CA8000的30%，cAMP的加入使其水平提高了5倍。然而，CA8306B的G3P脱氢酶诱导水平与CA8000相似，不受cAMP添加的影响。这些结果表明，glpK操纵子的促进需要cAMP，而glpD操纵子的促进则不需要。
The aerobic catabolism of glycerol depends on the expression of the glpK operon specifying a glycerol kinase and the glpD operon specifying an sn-glycerol-3-phosphate (G3P) dehydrogenase. It has not been clearly established how the expression of these operons is dependent on adenosine 3',5'-cyclic monophosphate (cAMP). We have isolated a promoterlike mutant (CA8306B) which, owing to a mutation in the glpK operon, can utilize glycerol in the absence of cAMP. Glycerol kinase and G3P dehydrogenase are inducible in CA8306B and its wild-type parent CA8000. The induced level of glycerol kinase in CA8306B is 30% that of CA8000 and this level is increased fivefold by the addition of cAMP. However, the induced level of G3P dehydrogenase in CA8306B is similar to that of CA8000 and is unaffected by cAMP addition. These results suggest that the promotion of the glpK operon requires cAMP whereas the promotion of the glpD operon does not.