The contribution of the nuclear reaction 1H(n, gamma)2D to the yield of DNA single strand breaks in cultured mammalian cells irradiated by thermal neutrons.
The contribution of the nuclear reaction 1H(n, gamma)2D to the yield of DNA single strand breaks in cultured mammalian cells irradiated by thermal neutrons.
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热中子辐照的培养哺乳动物细胞中核反应 1H(n, gamma)2D 对 DNA 单链断裂产量的贡献。
DOI：
10.1080/09553008514551601
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
M. Akaboshi
中科院分区：
文献类型：
--
作者：
H. Maki;M. Saito;K. Kawai;Y. Honda;M. Akaboshi
用碱性蔗糖梯度法测定了热中子辐照后HeLa S-3细胞DNA单链断裂率。1H（n，γ）2D反应的ssb的产率的贡献，确定通过取代D2 O为H2O在辐照介质中。计算表明，当细胞在H2O介质中辐照时，污染γ射线、核反应1H（n，γ）2D和其他核反应的贡献百分比分别为31%、44%和25%，假设效应是加和的。在相同的吸收剂量下，1H（n，gamma）2D核反应诱发的ssb数至少是60 Co γ射线诱发的ssb数的4.4倍。两种可能的解释进行了讨论，以解释高效率的1H（n，γ）2D反应的SSB诱导。
The yield of single strand breaks (ssb) in DNA of the HeLa S-3 cells after thermal neutron irradiation was examined using the alkaline sucrose gradient method. The contribution of the 1H(n, gamma)2D reaction to the yield of ssb was determined by substituting D2O for H2O in the irradiated medium. Calculation shows that when cells are irradiated in the H2O medium, the per cent contribution of the contaminating gamma-rays, the nuclear reaction 1H(n, gamma)2D and the other nuclear reactions is 31, 44 and 25 per cent respectively assuming additivity of effects. The estimated number of ssb induced by the nuclear reaction 1H(n, gamma)2D was at least 4.4 times greater than that by 60Co gamma-rays at the same absorbed dose. Two possible interpretations are discussed to explain the high efficiency of the 1H(n, gamma)2D reaction for ssb induction.