Preparation of Fluorescent Magnetic Nanodiamonds and Cellular Imaging
Preparation of Fluorescent Magnetic Nanodiamonds and Cellular Imaging
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DOI：
10.1021/ja804253y
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发表时间：
2008-11-19
影响因子：
15
通讯作者：
Chiang, Chi-Shiun
中科院分区：
文献类型：
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作者：
Chang, In Pin;Hwang, Kuo Chu;Chiang, Chi-Shiun
以纳米金刚石和二茂铁的混合粉末为原料，在微波聚焦炉中进行固态微波电弧放电制备磁性纳米金刚石。高分辨率透射电子显微镜（HRTEM）图像显示，磁性纳米金刚石是由纳米金刚石表面的石墨烯层包裹的铁纳米颗粒组成的。通过在磁性纳米金刚石表面接枝聚丙烯酸和荧光素邻甲基丙烯酸酯，对磁性纳米金刚石进行化学修饰，使其具有荧光特性。荧光磁性纳米金刚石是水溶性的，溶解度类似于2.1g/L。细胞成像实验表明，在不存在激动剂的情况下，荧光磁性纳米金刚石可以容易地被HeLa细胞摄取（即，叶酸）部分。
Magnetic nanodiamonds were prepared via solid-state microwave arcing of a nanodiamond-ferrocene mixed powder in a focused microwave oven. High-resolution transmission electron microscope (HRTEM) images show that a magnetic nanodiamond is composed of iron nanoparticles encapsulated by graphene layers on the surface of nanodiamonds. Fluorescence property was introduced onto magnetic nanodiamonds by chemical modification of magnetic nanodiamonds via surface grafting of poly(acrylic acids) and fluorescein o-methacrylate. Fluorescent magnetic nanodiamonds are water soluble with a solubility of similar to 2.1 g/L. Cellular-imaging experiments show that fluorescent magnetic nanodiamonds could be ingested by HeLa cells readily in the absence of agonist (i.e., folate) moieties on the surface of nanodiamonds.