lncRNA GAS5 Is Upregulated in Osteoporosis and Down regulates miR-21 to Promote Apoptosis of Osteoclasts
lncRNA GAS5 Is Upregulated in Osteoporosis and Down regulates miR-21 to Promote Apoptosis of Osteoclasts
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DOI：
10.2147/cia.s235197
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发表时间：
2020-01-01
影响因子：
3.6
通讯作者：
Zhu, Liyu
中科院分区：
文献类型：
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作者：
Cong, Chunlei;Tian, Jun;Zhu, Liyu
背景：已有报道LncRNA生长停滞特异性转录本5(Gas5)与miR-21相互作用，miR-21在骨质疏松症中起关键作用。本研究旨在探讨Gas5在骨质疏松中的作用。方法：采用RT-qPCR方法检测骨质疏松症患者和正常对照组血浆中Gas5和miR-21的表达水平。用ROC曲线分析Gas5、miR-21对骨质疏松症的诊断价值。采用过表达实验评估Gas5与miR-21之间的相互作用。结果：本研究旨在探讨Gas5与miR-21在骨质疏松中的作用。结果表明，骨质疏松患者血浆中Gas5表达上调，miR-21表达下调。骨质疏松症患者的血浆样本中Gas5和miR-21的表达水平呈负相关，而对照组的血浆样本中Gas5和miR-21的表达水平没有相关性。骨质疏松症患者外周血中Gas5和miR-21的表达变化。在破骨细胞中，Gas5的过表达导致miR-21表达下调，而miR-21的过表达不影响Gas5的表达。Gas5过表达促进破骨细胞凋亡，miR-21过表达抑制破骨细胞凋亡。此外，miR-21的过表达减弱了Gas5过表达对细胞凋亡的促进作用。结论：Gas5在骨质疏松中上调，可能通过下调miR-21促进破骨细胞的凋亡。
Background: It has been reported that lncRNA growth arrest-specific transcript 5 (GAS5) interacts with miR-21, which plays critical roles in osteoporosis. The involvement of GAS5 in osteoporosis was investigated in this study.Methods: Expression levels of GAS5 and miR-21 in plasma of both osteoporosis patients and healthy controls were determined by RT-qPCR. Diagnostic values of GAS5 and miR-21 for osteoporosis were analyzed by ROC curve analysis. Overexpression experiments were used to assess the interactions between GAS5 and miR-21. The roles of GAS5 and miR-21 in the apoptosis of osteoclasts were investigated by cell apoptosis assay.Results: The present study aimed to investigate the roles of GAS5 in osteoporosis. The results showed that GAS5 was upregulated, while miR-21 was downregulated in plasma of osteoporosis patients. Expression levels of GAS5 and miR-21 were inversely con elated across plasma samples from osteoporosis patients but not the plasma samples from the controls. Altered expression of GAS5 and miR-21 distinguished osteoporosis patients from the controls. In osteoclasts, overexpression of GAS5 led to downregulation of miR-21, while overexpression of miR-21 did not affect the expression of GAS5. Overexpression of GAS5 led to promoted apoptosis of osteoclasts, while overexpression of miR-21 led to suppressed apoptosis of osteoclasts. In addition, overexpression of miR-21 attenuated the enhancing effects of overexpressing GAS5 on cell apoptosis.Conclusion: GAS5 is upregulated in osteoporosis and may downregulate miR-21 to promote the apoptosis of osteoclasts.