Uncultured bacterial diversity in a seawater recirculating aquaculture system revealed by 16S rRNA gene amplicon sequencing
Uncultured bacterial diversity in a seawater recirculating aquaculture system revealed by 16S rRNA gene amplicon sequencing
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DOI：
10.1007/s12275-016-5571-4
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发表时间：
2016-04
影响因子：
3
通讯作者：
Daeun Lee;Jinhwan Lee;Young-Mog Kim;J. Myeong;Kyoung-Ho Kim
中科院分区：
文献类型：
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作者：
Daeun Lee;Jinhwan Lee;Young-Mog Kim;J. Myeong;Kyoung-Ho Kim
采用16S rRNA扩增子测序技术对海水循环水养殖系统（RAS）中的细菌多样性进行了研究，以了解细菌群落在系统中的作用。RAS在温度（15°C、20°C和25°C）和盐度（20‰、25‰和32.5‰）的9种不同组合下工作。每种情况下从5或6个RAS槽（生物过滤器）中收集样本。对50个样本进行了分析。变形杆菌和拟杆菌最常见（两门总数67.2% ~ 99.4%），两者比例成反比。平均≥1%的细菌包括放线菌门（2.9%）、植物菌门（2.0%）、硝化螺旋菌门（1.5%）和酸杆菌门（1.0%）；它们优先存在于填充床生物滤池、网状生物滤池和成熟生物滤池中。3种生物滤池在门和操作分类单位（OTU）水平上的多样性均高于其他RAS池（曝气生物滤池、浮床生物滤池和鱼缸）。根据细菌群落将样品分成几组。主要分类类群红杆菌科和黄杆菌科分布广泛。一些分类类群如[腐生菌科]、胞噬科、八足菌科和marivita等呈簇状分布。在低温下大量检测到与phaeobacteria和sediminicola相关的reads。3个生物滤池中均检测到大量且频繁的硝化细菌。系统发育分析表明，通过生物过滤器可以广泛观察到几种相似的otu。除了变形杆菌和拟杆菌外，不同的细菌群落和较小的分类类群似乎对RAS的硝化活性起着重要作用，并且似乎是必需的，特别是在填充床生物过滤器，网状生物过滤器和成熟生物过滤器中。
Bacterial diversity in a seawater recirculating aquaculture system (RAS) was investigated using 16S rRNA amplicon sequencing to understand the roles of bacterial communities in the system. The RAS was operated at nine different combinations of temperature (15°C, 20°C, and 25°C) and salinity (20‰, 25‰, and 32.5‰). Samples were collected from five or six RAS tanks (biofilters) for each condition. Fifty samples were analyzed.ProteobacteriaandBacteroideteswere most common (sum of both phyla: 67.2% to 99.4%) and were inversely proportional to each other. Bacteria that were present at an average of ≥ 1% included Actinobacteria (2.9%) Planctomycetes (2.0%), Nitrospirae (1.5%), and Acidobacteria (1.0%); they were preferentially present in packed bed biofilters, mesh biofilters, and maturation biofilters. The three biofilters showed higher diversity than other RAS tanks (aerated biofilters, floating bed biofilters, and fish tanks) from phylum to operational taxonomic unit (OTU) level. Samples were clustered into several groups based on the bacterial communities. Major taxonomic groups related to familyRhodobacteraceaeandFlavobacteriaceaewere distributed widely in the samples. Several taxonomic groups like [Saprospiraceae],Cytophagaceae,Octadecabacter, andMarivitashowed a cluster-oriented distribution.PhaeobacterandSediminicola-related reads were detected frequently and abundantly at low temperature. Nitrifying bacteria were detected frequently and abundantly in the three biofilters. Phylogenetic analysis of the nitrifying bacteria showed several similar OTUs were observed widely through the biofilters. The diverse bacterial communities and the minor taxonomic groups, except forProteobacteriaandBacteroidetes, seemed to play important roles and seemed necessary for nitrifying activity in the RAS, especially in packed bed biofilters, mesh biofilters, and maturation biofilters.