Plasmid-encoded regulator of extracellular proteases in Bacillus anthracis.
Plasmid-encoded regulator of extracellular proteases in Bacillus anthracis.
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炭疽芽孢杆菌胞外蛋白酶的质粒编码调节剂。
DOI：
10.1128/jb.187.9.3133-3138.2005
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Fulroth,Ben
中科院分区：
文献类型：
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作者：
Aronson,ArthurI;Bell,Chris;Fulroth,Ben
pXO1质粒的炭疽芽孢杆菌Sterne在感染后阶段分泌的蛋白酶活性增强，但溶血或卵磷脂酶活性没有变化。一个酶原档案显示至少有六种蛋白酶，包括丝氨酸，金属，也许半胱氨酸类型。有相似的蛋白酶分泌量的密切相关的物种蜡状芽孢杆菌和苏云金芽孢杆菌，但模式不同。在pXO 1质粒编码的蛋白质中，有一个被命名为Cot 43的四肽蛋白，它与枯草芽孢杆菌的Rap蛋白和B.苏云金杆菌一个破坏的thecot43基因导致几种蛋白酶的过量生产，在某种程度上比质粒治愈的菌株。用cot43基因的克隆转化这些菌株中的任何一种，导致抑制某些蛋白酶的积累和诱导至少一种蛋白酶。在acZ融合的基础上，在蛋白酶积累的时候，在指数晚期细胞中，cot43基因的转录增加。将acZ融合蛋白表达到两个B. cot 43被破坏的菌株中的炭疽细胞外蛋白酶基因比Sterne菌株中的更大，表明在转录水平上的调节。InB.炭疽杆菌，pXO1质粒编码的蛋白质直接调节或间接调节几种染色体编码的细胞外蛋白酶的基因转录。
Bacillus anthracisSterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protease secreted by the closely related speciesBacillus cereusandBacillus thuringiensis, but the patterns differed. Among the pXO1 plasmid-encoded proteins, there is a tetratricopeptide protein designated Cot43 that is related to the Rap proteins ofBacillus subtilisand the PlcR pleiotropic regulator of secreted enzymes and toxins inB. thuringiensis. A disruption of thecot43gene resulted in overproduction of several proteases to a somewhat greater extent than in the plasmid-cured strain. Transformation of either of these strains with a clone of thecot43gene resulted in the inhibition of accumulation of some of the proteases and induction of at least one. On the basis oflacZfusions, transcription of thecot43gene increased in late exponential cells at the time of protease accumulation. The expression oflacZfusions to the upstream regions of twoB. anthracisextracellular protease genes was greater in the strain with the disruption ofcot43than in the Sterne strain, indicating regulation at the level of transcription. InB. anthracis, a pXO1 plasmid-encoded protein directly modulates or indirectly regulates the transcription of genes for several chromosomally encoded extracellular proteases.