In vitro heart valve tissue engineering.
In vitro heart valve tissue engineering.
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DOI：
10.1007/978-1-59745-443-8_18
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
D. Schmidt;A. Mol;J. Kelm;S. Hoerstrup
中科院分区：
文献类型：
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作者：
D. Schmidt;A. Mol;J. Kelm;S. Hoerstrup
心脏瓣膜置换术是终末期心脏瓣膜病最常见的外科治疗方法。所有当代心脏瓣膜置换术的一个共同的主要缺点是缺乏生长、修复和重塑能力。为了克服这些局限性，组织工程学的新兴领域正专注于体外生成具有功能的、活的心脏瓣膜替换物。基本方法使用脱细胞异种或生物聚合物材料的起始矩阵，这些材料配置成心脏瓣膜的形状，并随后进行细胞接种。此外，在生物反应器系统中使用机械加载的体外策略已经被开发出来以促进组织成熟。本章简要概述了目前的概念，并提供了体外心脏瓣膜组织工程的详细方法。
Heart valve replacement represents the most common surgical therapy for end-stage valvular heart diseases. A major drawback all contemporary heart valve replacements have in common is the lack of growth, repair, and remodeling capabilities. To overcome these limitations, the emerging field of tissue engineering is focusing on the in vitro generation of functional, living heart valve replacements. The basic approach uses starter matrices of either decellularized xenogeneic or biopolymeric materials configured in the shape of the heart valve and subsequent cell seeding. Moreover, in vitro strategies using mechanical loading in bioreactor systems have been developed to improve tissue maturation. This chapter gives a short overview of the current concepts and provides detailed methods for in vitro heart valve tissue engineering.