Exploring Grass Morphology & Mutant Phenotypes Using Setaria viridis
Exploring Grass Morphology & Mutant Phenotypes Using Setaria viridis
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DOI：
10.1525/abt.2021.83.5.311
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发表时间：
2021-06
期刊：
影响因子：
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通讯作者：
R. Kaggwa;Hui Jiang;Rita A. Ryan;Justin Paul Zahller;E. Kellogg;T. Woodford-Thomas;Kristine Callis‐Duehl
中科院分区：
文献类型：
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作者：
R. Kaggwa;Hui Jiang;Rita A. Ryan;Justin Paul Zahller;E. Kellogg;T. Woodford-Thomas;Kristine Callis‐Duehl
摘要 在全球范围内，人类摄入的大部分热量来自禾本科（Poaceae）作物，包括甘蔗（Saccharum spp.）、水稻（Oryza sativa）、玉米（或玉米，Zea mays）和小麦（Triticum aestivum）。这些草具有独特的形态和花序结构，有些还进化出了一种不常见的光合作用途径，赋予干旱和耐热性，即 C4 途径。大多数中学生不了解这些作物的全球价值，也不熟悉植物科学基础知识，例如草结构以及基因型和表型的遗传概念。绿谷子 (Setaria viridis) 是 C4 植物的模式生物，也是包括甘蔗在内的全球重要禾本科植物的近亲。它非常适合关于草形态、草的经济价值和 C4 光合作用途径的教学。本文详细介绍了一个教学模块，该模块使用 S. viridis 通过草形态、生长周期和遗传学的实验室调查，让整个课堂的学生参与真实的研究。该模块包括协议和作业，指导学生完成一代 S. viridis 突变体的生长过程，并参考野生型植物从种子到种子的过程，进行测量，对突变体表型进行批判性观察，并讨论其生理学意义。
Abstract Globally, most human caloric intake is from crops that belong to the grass family (Poaceae), including sugarcane (Saccharum spp.), rice (Oryza sativa), maize (or corn, Zea mays), and wheat (Triticum aestivum). The grasses have a unique morphology and inflorescence architecture, and some have also evolved an uncommon photosynthesis pathway that confers drought and heat tolerance, the C4 pathway. Most secondary-level students are unaware of the global value of these crops and are unfamiliar with plant science fundamentals such as grass architecture and the genetic concepts of genotype and phenotype. Green foxtail millet (Setaria viridis) is a model organism for C4 plants and a close relative of globally important grasses, including sugarcane. It is ideal for teaching about grass morphology, the economic value of grasses, and the C4 photosynthetic pathway. This article details a teaching module that uses S. viridis to engage entire classrooms of students in authentic research through a laboratory investigation of grass morphology, growth cycle, and genetics. This module includes protocols and assignments to guide students through the process of growing one generation of S. viridis mutants and reference wild-type plants from seed to seed, taking measurements, making critical observations of mutant phenotypes, and discussing their physiological implications.