Genetic analysis and transcriptome analysis of the mini mutant of the silkworm, Bombyx mori.
Genetic analysis and transcriptome analysis of the mini mutant of the silkworm, Bombyx mori.
复制标题
家蚕微小突变体的遗传分析和转录组分析。
DOI：
10.1002/arch.21774
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发表时间：
2021
影响因子：
2.2
通讯作者：
Qiaoling Zhao
中科院分区：
文献类型：
--
作者：
Mengjie Gao;Xinglin Mei;Cong Li;Pengcheng Yu;Dongxu Shen;Qiaoling Zhao
家蚕在不同生长发育阶段，某些内源基因的表达水平、蛋白酶活性以及信号通路的调控均存在差异。从家蚕正常品系S8V中发现了突变体mini，其体型从二龄开始小于野生型，以后差异更加显著。本研究对该突变体的遗传分析表明，该突变体受单隐性基因控制，表现为纯合致死。转录组分析结果表明，在家蚕二龄48 h内共检测到2944个差异表达基因，其中1638个基因表达上调，1306个基因表达下调。这些DEG主要分布在生物学过程、细胞组分和分子功能中。基于KEGG数据库的功能注释显示，这些基因主要集中在真核生物的代谢途径、脂肪酸代谢途径、核糖体生物合成等方面，进一步分析发现，与生长代谢相关的酶基因、保幼激素、蜕皮激素等基因在转录水平上存在差异。这些结果提供了新的实验证据有关的机制，潜在的微型突变体的形成。
The expression levels of some intrinsic genes, protease activity, and regulation of signaling pathways were distinct during different growth and development stages in the silkworm, Bombyx mori. The silkworm mutant mini was discovered from the normal silkworm strain S8V, and the body-size of the mini mutant was smaller than the wild-type from the second-instar and the difference became more significant in the following stages. In this study, genetic analysis of mini mutant showed that mini mutant was controlled by a single recessive gene, manifested as homozygous lethal. Then, the transcriptome analysis of the mini mutant indicated that 2944 differentially expressed genes (DEGs) were identified from the silkworm in the 48 h of the second-instar, of which 1638 genes in the mini mutants were upregulated and 1306 genes were downregulated. These DEGs were mainly distributed in the biological process, cellular component, and molecular function. The functional annotation based on the KEGG database showed that these genes were mainly clustered in metabolic pathways, fatty acid metabolism pathways, ribosome biogenesis in eukaryotes, and so on. Further analysis indicated that some genes involved in the growth and metabolism including enzyme genes, juvenile hormone, and ecdysone exhibited different transcriptional levels. These results provided new experimental evidence regarding the mechanism of the underlying formation of mini mutants.