Hypocorticism and interrenal hyperplasia are not directly related to masculinization in XX mas-1/mas-1 carp, Cyprinus carpio
Hypocorticism and interrenal hyperplasia are not directly related to masculinization in XX mas-1/mas-1 carp, Cyprinus carpio
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DOI：
10.1016/j.ygcen.2005.02.024
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发表时间：
2005-08-01
影响因子：
2.7
通讯作者：
Komen, J
中科院分区：
文献类型：
--
作者：
Ruane, NM;Lambert, JGD;Komen, J
本研究报告了一个纯合子XX公鲤品系（E5），它不能安装正常的皮质醇应激反应。早期的经典遗传学分析表明，E5期鱼的雄性化是由一个假定的隐性突变（mas（-1）/1 mas（-1））引起的。研究了E5期鱼类的皮质功能低下是否与雄性化有关。头肾组织分离E5鱼表现出较低的皮质醇生产能力，在体外，也表现出减少的敏感性刺激与ACTH相比，同基因XY雄性鲤鱼品系（STD）。头肾组织体外雄激素分泌无品系差异。E5鱼表现出显着增生的肾间组织（肾上腺同源的硬骨鱼）位于头肾。与STD匀浆相比，E5头-肾匀浆中的双烯醇酮转化率显著较低，然而，在17 α-羟孕酮转化为皮质醇中未发现菌株差异。性腺匀浆孵育与异烯醇酮显示没有应变差异转化为皮质类固醇和雄激素。结果表明，该品系鲤鱼肾间组织增生和皮质功能减退可能是由于肾间组织中P450 c17酶的17 α-羟化酶活性下降所致，但这一缺陷可能不是导致XX基因型雄性化的主要因素。(c)2005年爱思唯尔公司All rights reserved.
This study reports on a homozygous XX male strain of common carp (E5), which fail to mount a normal cortisol stress response. Earlier classical genetic analysis had indicated that masculinization of E5 fish was caused by a putative recessive mutation (mas(-1)/1mas(-1)). Hypocorticism in E5 fish was studied to investigate if it was related to masculinization. Head-kidney tissues isolated from E5 fish showed a low cortisol-producing capacity in vitro, and also demonstrated a reduced sensitivity to stimulation with ACTH, when compared with an isogenic XY male carp strain (STD). There was no strain difference in androgen production by head-kidney tissues in vitro. E5 fish exhibited significant hyperplasia of the interrenal tissue (adrenal homologue of teleost fish) located in the head-kidney. Conversion of pregnenolone was significantly lower in E5 head-kidney homogenates, compared to STD homogenates, however, no strain difference was found in the conversion of 17 alpha-hydroxyprogesterone into cortisol. Gonad homogenates incubated with pregnenolone showed no strain difference in conversion to corticosteroids and androgens. Results indicate that the interrenal hyperplasia and hypocorticism in this strain of carp may be due to a dysfunction of the 17 alpha-hydroxylase activity of the enzyme P450c17 in the interrenal, but that this defect may not be the primary factor resulting in masculinization of these XX genotypes. (c) 2005 Elsevier Inc. All rights reserved.