Molecular cloning and expression analysis of a gene for a sucrose transporter maize (Zea mays L.)
Molecular cloning and expression analysis of a gene for a sucrose transporter maize (Zea mays L.)
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玉米（Zea mays L.）蔗糖转运体基因的分子克隆和表达分析
DOI：
10.1093/oxfordjournals.pcp.a029489
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发表时间：
1999-10-01
影响因子：
4.9
通讯作者：
Ohsugi, R
中科院分区：
文献类型：
--
作者：
Aoki, N;Hirose, T;Ohsugi, R
本文报道了从玉米（Zea mays L.）和基因表达的分析。一个cDNA克隆（ZmSUT1）被鉴定为蔗糖转运蛋白cDNA，这是由于其在氨基酸水平上与已从其它高等植物物种克隆的蔗糖转运蛋白的序列同源性。基于基因组Southern杂交的结果，ZmSUT1似乎是一个单拷贝基因。幼苗和叶片的北方印迹分析表明，蔗糖转运蛋白参与了从源叶片输出积累的碳水化合物。从成熟叶片中的转录水平和碳水化合物含量的测量，我们提出，玉米蔗糖转运蛋白基因的表达是由玉米源叶中的可溶性糖，如蔗糖和葡萄糖的量正调控。此外，基于基因在生殖阶段玉米植物中表达的组织特异性，蔗糖转运蛋白可能在库组织如花梗以及源组织如叶片中起作用。
Here we report the cloning of a sucrose transporter cDNA from maize (Zea mays L.) and an analysis of the expression of the gene. A cDNA clone (ZmSUT1) was identified as a sucrose transporter cDNA from its sequence homology at the amino acid level to sucrose transporters that have been cloned from other higher plant species. Based on the results of genomic Southern hybridization, ZmSUT1 appears to be a single copy gene. A Northern blot analysis of seedlings and leaf blades suggests that the sucrose transporter is involved in the export of accumulated carbohydrates from source leaf blades. From the measurements of transcript levels and carbohydrate contents in mature leaf blades, we propose that the expression of the gene for the maize sucrose transporter is positively regulated by the amounts of soluble sugars such as sucrose and glucose in source leaves of maize. In addition, based on the tissue specificity of the expression of the gene in maize plants at the reproductive stage, it is possible that the sucrose transporter acts in sink tissues such as pedicels as well as in source tissues such as leaf blades.