Orphan receptor TR3 is essential for the maintenance of stem-like properties in gastric cancer cells
Orphan receptor TR3 is essential for the maintenance of stem-like properties in gastric cancer cells
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孤儿受体TR3对于维持胃癌细胞的干细胞样特性至关重要
DOI：
10.1016/j.canlet.2012.09.022
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发表时间：
2013-02-01
期刊：
影响因子：
9.7
通讯作者：
Cai, Jian-chun
中科院分区：
文献类型：
--
作者：
Zhan, Yan-yan;He, Jian-ping;Cai, Jian-chun
孤儿受体TR3是细胞增殖和凋亡的重要调节因子。然而，TR3是否参与调节癌细胞的干细胞样特性仍不清楚。本研究表明TR3表达在胃肿瘤球细胞中增加，并且与癌干细胞（CSC）特征呈正相关。敲除 TR3 会导致其在胃癌细胞和肿瘤球细胞中的干细胞样特性受到抑制。这个过程涉及到sternness相关基因Oct-4和Nanog以及侵袭相关基因MMP-9表达的降低。我们进一步确定 Nanog 是转录因子 TR3 的新靶标。总之，这些数据首次证明 TR3 对于维持人胃癌细胞的干细胞样特性至关重要，并暗示 TR3 作为胃癌的新治疗靶点。 (c) 2012 Elsevier Ireland Ltd. 保留所有权利。
The orphan receptor TR3 is an important regulator of cell proliferation and apoptosis. However, whether TR3 is involved in regulating the stem-like properties of cancer cells remains unknown. The present study shows that TR3 expression is increased in gastric tumorsphere cells and is positively correlated with cancer stern cell (CSC) characteristics. Knocking down TR3 leads to the suppression of its stem-like properties in both gastric cancer cells and tumorsphere cells. This process involves the decreased expression of the sternness-related genes Oct-4 and Nanog and the invasion-related gene MMP-9. We further identify Nanog as a new target for the transcription factor TR3. Together, these data demonstrate for the first time that TR3 is essential for the maintenance of stem-like properties in human gastric cancer cells and implicate TR3 as a new therapeutic target for gastric cancer. (c) 2012 Elsevier Ireland Ltd. All rights reserved.