Brazilian Propolis: Seasonal Variation of the Prenylated p-Coumaric Acids and Antimicrobial Activity
Brazilian Propolis: Seasonal Variation of the Prenylated p-Coumaric Acids and Antimicrobial Activity
复制标题
DOI：
10.1080/13880200802370373
复制
发表时间：
2008-12-01
影响因子：
3.8
通讯作者：
Silva, M. L. A.
中科院分区：
文献类型：
--
作者：
Jorge, R.;Furtado, N. A. J. C.;Silva, M. L. A.
巴西绿色蜂胶用于食品和饮料中，以改善健康和预防疾病，具有抗氧化、抗诱变和抗菌活性。大多数生物活性被认为与高水平的核糖苷、青蒿素C和糖精有关，这些化合物也存在于龙珠酒中（菊科）。由于蜂胶的化学成分取决于采集的地区和时间，以及其植物来源，因此研究了季节变化对四个不同地点巴西蜂胶的对香豆酸含量和体外抗菌活性的影响。结果表明，蜂胶样品对金黄色葡萄球菌和嗜根葡萄球菌的MIC值在100 ~ 300 μ g/mL之间，而对铜绿假单胞菌、大肠杆菌和白色念珠菌均无活性。高效液相色谱分析表明，不同年份、不同研究地点的芦丁苷（drupanin）、青蒿素C （artepillin C）和糖精（baccharin）含量存在差异。此外，我们还认为在位点1和位点2中，龙珠酒是巴西蜂胶的主要植物来源，而在位点3和位点4中，其他植物也被蜜蜂用来生产蜂胶。不同年份蜂胶样品的抑菌活性相似，但对香豆酸的含量不同。
Brazilian green propolis, which is used in food and beverages to improve health and to prevent diseases, demostrates antioxidant, antimutagenic, and antimicrobial activities. Most biological activities are thought to be related to the high levels of drupanin, artepillin C, and baccharin, which are compounds also present in Baccharis dracunculifolia D.C. (Asteraceae). Since propolis chemical composition depends on the region and the period of collection, as well as its plant origin, the effect of seasonal variation on the both content of prenylated p-coumaric acids and in vitro antimicrobial activity of Brazilian propolis from four different sites, was performed. The results showed that MIC values ranged from 100 to 300 mu g/mL against both Staphylococcus aureus and Kocuria rhizophila, while none of the propolis samples was active against Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. HPLC analysis showed that the content of drupanin, artepillin C, and baccharin varied throughout the year, as well as among the different study sites. Also, it is suggested that Baccharis dracunculifolia is the main botanical source of Brazilian propolis in sites I and 2, while in sites 3 and 4, other plant species are also used by bees to produce propolis. All the evaluated propolis samples exhibited similar antibacterial activity, but different contents of prenylated p-coumaric acids throughout the year.