Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin.
Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin.
复制标题
DOI：
10.2147/ijn.s20413
复制
发表时间：
2011
影响因子：
8
通讯作者：
Cui Z
中科院分区：
文献类型：
--
作者：
Kumar A;Li X;Sandoval MA;Rodriguez BL;Sloat BR;Cui Z
本研究旨在评估微针预处理在多大程度上能在体外和体内促进纳米粒的皮肤渗透。还研究了活细菌，即物理上的纳米颗粒或微粒子，通过微针处理的小鼠皮肤的渗透，以评估微生物感染的潜在风险。研究发现，用微针对小鼠皮肤进行预处理，可以使230 nm大小的固体脂质纳米粒与卵清蛋白结合在其表面进行渗透。用卵白蛋白纳米颗粒的微针对小鼠皮肤区域进行经皮免疫比单独使用卵白蛋白诱导更强的抗卵白蛋白抗体反应。卵白蛋白抗原的剂量决定了微针介导的卵白蛋白纳米粒经皮免疫是否比皮下注射相同的卵白蛋白纳米粒诱导了更强的免疫应答。微针治疗允许活体大肠杆菌的皮肤渗透，但渗透的程度不大于皮下注射。用纳米颗粒携带的抗原对微针治疗的皮肤区域进行经皮免疫可以潜在地诱导强烈的免疫反应，而与微针治疗相关的细菌感染的风险并不比皮下注射更大。
The present study was designed to evaluate the extent to which pretreatment with microneedles can enhance skin permeation of nanoparticles in vitro and in vivo. Permeation of live bacteria, which are physically nanoparticles or microparticles, through mouse skin pretreated with microneedles was also studied to evaluate the potential risk of microbial infection. It was found that pretreatment of mouse skin with microneedles allowed permeation of solid lipid nanoparticles, size 230 nm, with ovalbumin conjugated on their surface. Transcutaneous immunization in a mouse skin area pretreated with microneedles with ovalbumin nanoparticles induced a stronger antiovalbumin antibody response than using ovalbumin alone. The dose of ovalbumin antigen determined whether microneedle-mediated transcutaneous immunization with ovalbumin nanoparticles induced a stronger immune response than subcutaneous injection of the same ovalbumin nanoparticles. Microneedle treatment permitted skin permeation of live Escherichia coli, but the extent of the permeation was not greater than that enabled by hypodermic injection. Transcutaneous immunization on a microneedle-treated skin area with antigens carried by nanoparticles can potentially induce a strong immune response, and the risk of bacterial infection associated with microneedle treatment is no greater than that with a hypodermic injection.