MONOCLONAL-ANTIBODIES WITH SELECTIVE SPECIFICITY FOR ALZHEIMER TAU ARE DIRECTED AGAINST PHOSPHATASE-SENSITIVE EPITOPES
MONOCLONAL-ANTIBODIES WITH SELECTIVE SPECIFICITY FOR ALZHEIMER TAU ARE DIRECTED AGAINST PHOSPHATASE-SENSITIVE EPITOPES
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DOI：
10.1007/bf00227819
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发表时间：
1992-08-01
影响因子：
12.7
通讯作者：
GHEUENS, J
中科院分区：
文献类型：
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作者：
MERCKEN, M;VANDERMEEREN, M;GHEUENS, J
微管相关蛋白Tau的一种修饰形式是阿尔茨海默病中发现的成对螺旋丝（PHF）的主要成分。这些翻译后Tau修饰的表征由于缺乏足够的PHF-Tau特异性标志物而受阻。在这里，我们描述了几种单克隆抗体，制备与PHF免疫，其中两个显示PHF-Tau没有交叉反应性与正常的Tau的选择性特异性。这两个单克隆抗体识别的表位对碱性磷酸酶处理敏感。在Western印迹中，这些单克隆抗体与阿尔茨海默病相关PHF-Tau上的异常磷酸化表位特异性反应。其中一种新抗体可用于构建夹心酶联免疫吸附测定，用于特异性检测PHF-Tau，而与正常Tau蛋白无交叉反应性。
A modified form of the microtubule-associated protein Tau is the major component of the paired helical filaments (PHF) found in Alzheimer's disease. The characterization of these posttranslational Tau modifications is hindered by the lack of sufficient PHF-Tau-specific markers. Here we describe several monoclonal antibodies, prepared by immunization with PHF, two of which showed a selective specificity for PHF-Tau without cross-reactivity with normal Tau. Epitope recognition by these two monoclonals was sensitive to alkaline phosphatase treatment. In Western blotting these monoclonal antibodies reacted specifically with the abnormally phosphorylated epitopes on Alzheimer's disease-associated PHF-Tau. One of the new antibodies can be used for the construction of a sandwich enzyme-linked immunosorbent assay for the specific detection of PHF-Tau without cross-reactivity to normal Tau proteins.