Expression of the whey acidic protein in transgenic pigs impairs mammary development
Expression of the whey acidic protein in transgenic pigs impairs mammary development
复制标题
转基因猪中乳清酸性蛋白的表达损害乳腺发育
作者：
A. Shamay;V. Pursel;Erby Wilkinson;R. Wall;L. Hennighausen
在小鼠、大鼠、兔和骆驼的乳汁中发现了乳清酸性蛋白，其基因在妊娠晚期和整个哺乳期的乳腺组织中特异性表达。乳清酸性蛋白的一个特征是“四个二硫键核心”特征，其也存在于参与器官发育的蛋白质中。我们已经产生了六个行的转基因猪携带小鼠乳清酸性蛋白转基因，并在其乳腺中高水平表达。来自三个品系的转基因母猪不能产生足够量的奶来支持健康后代的正常发育。这种表型似乎与表达乳清酸性蛋白转基因的小鼠所表现出的乳杆菌表型相似，如果不相同的话。产后泌乳母猪的乳腺组织具有不成熟的组织学外观，这与正常发育或退化期间观察到的组织学外观不同。乳清酸性蛋白转基因的表达被发现在性不成熟的猪的乳腺组织中，但在母猪的泌乳能力正常的品系中没有。我们认为，过早合成乳清酸性蛋白损害乳房发育和功能。由于乳清酸性蛋白表达的不适当时机导致的乳房发育受损与具有“四个二硫键核心”特征的蛋白质参与组织形成的概念一致。
The whey acidic protein has been found in milk of mice, rats, rabbits and camels, and its gene is expressed specifically in mammary tissue at late pregnancy and throughout lactation. A characteristic of whey acidic protein is the ‘four-disulfide-core’ signature which is also present in proteins involved in organ development. We have generated six lines of transgenic pigs which carry a mouse whey acidic protein transgene and express it at high levels in their mammary glands. Transgenic sows from three lines could not produce sufficient quantities of milk to support normal development of healthy offspring. This phenotype appears to be similar, if not identical, to themilchlos phenotype exhibited by mice expressing whey acidic protein transgenes. Mammary tissue from post-partummilchlos sows had an immature histological appearance, which was distinct from that observed during normal development or involution. Expression of the whey acidic protein transgene was found in mammary tissue from sexually immature pigs frommilchlos lines, but not in sows from lines that appeared to lactate normally. We suggest that precocious synthesis of whey acidic protein impairs mammary development and function. Impaired mammary development due to inappropriate timing of whey acidic protein expression is consistent with the notion that proteins with the ‘four-disulfide-core’ signature participate in tissue formation.
大鼠酪蛋白基因表达的复杂激素调节。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hobbs,AA;Richards,DA;Kessler,DJ;Rosen,JM
通讯作者：
Rosen,JM
DOI：
10.1073/pnas.88.5.1701
发表时间：
1991-03-01
影响因子：
11.1
作者：
KEARSE, KP;HART, GW
通讯作者：
HART, GW