HIV type 1 in Thailand, 1994-1995: Persistence of two subtypes with low genetic diversity
HIV type 1 in Thailand, 1994-1995: Persistence of two subtypes with low genetic diversity
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DOI：
10.1089/aid.1998.14.319
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发表时间：
1998-03-01
影响因子：
1.5
通讯作者：
Schochetman, G
中科院分区：
文献类型：
--
作者：
Subbarao, S;Limpakarnjanarat, K;Schochetman, G
人类免疫缺陷病毒1型（HIV-1）在泰国的广泛传播始于1988年，到1994年估计累计感染人数为80万。在1994年和1995年期间，我们从泰国所有四个地区的九个地点收集了215名具有各种危险行为的无症状HIV-1感染者的血液标本，采用DNA直接测序（n = 95）、亚型特异性寡核苷酸探针检测（n = 201）和V3环肽酶免疫分析（PEIA）（n = 214）对214株HIV-1亚型和遗传异质性进行了测定。（175; 81.8%）或B B亚型37株B'型占94.9%，（5.1%）为较典型的北美样B型菌株（泰国的大多数亚型B毒株是系统发生树上亚型B分支内的不同亚群的一部分，称为B ';以前称为泰国B或B-B）在来自所有地区有危险性行为的人的149种病毒中，65例静脉吸毒者（IDUs）分离到的病毒中，E亚型占98.0%（29 E亚型占44.6%，B亚型占55.4%，其中B'亚型35株，E、B'亚型在吸毒人群中的分布存在地区差异，env基因V3和侧翼区的亚型内核苷酸差异（C2中段至V4区开始）较低（E亚型5.7%，B '亚型3.1%）与其他来自不同国家的HIV-1 M组亚型相比，两种亚型异质性低的这些发现表明，泰国可能是评价候选HIV-1疫苗的理想环境。注射吸毒者中E和B'亚型毒株的混合也为研究两种亚型之间的表型差异提供了机会。
Extensive transmission of human immunodeficiency virus type 1 (HIV-1) in Thailand began in 1988, resulting in an estimated 800,000 cumulative infections by 1994, During 1994 and 1995, we collected blood specimens from 215 asymptomatic HIV-1-infected people with various risk behaviors from nine locations in all four regions of Thailand, HIV-1 subtypes and genetic heterogeneity were determined for 214 strains by a combination of direct DNA sequencing (n = 95), subtype-specific oligonucleotide probe testing (n = 201), and V3-loop peptide enzyme immunoassay (PEIA) (n = 214), All strains were either env subtype E (175; 81.8%) or B (39; 18.2%), Of the subtype B isolates, 37 (94.9%) were B' and 2 (5.1%) were more typical North American-like B strains (most subtype B strains in Thailand are part of a distinct subcluster within the subtype B branch on phylogenetic trees, termed B'; formerly Thai B or B-B) Of 149 viruses from people with sexual risk behaviors from all regions, 146 (98.0%) were subtype E, Of 65 viruses from injecting drug users (IDUs), 29 (44.6%) were subtype E and 36 (55.4%) were subtype B, including 35 B' strains, There was regional variation in the proportions of subtypes E and B' among IDUs, The intrasubtype nucleotide divergence within the V3 and flanking regions of the env gene (mid-C2 to the start of the V4 region) was low (5.7% for subtype E and 3.1% for subtype B') compared with other HIV-1 group M subtypes from different countries, These findings of two subtypes with low heterogeneity indicate that Thailand may be a desirable setting for evaluating candidate HIV-1 vaccines, The mix of subtype E and B' strains among IDUs also offers the opportunity to study phenotypic differences between the two subtypes.