Preparation of L-α-glyceryl phosphorylcholine by hydrolysis of soy lecithin using phospholipase A1 in a novel solvent-free water in oil system
Preparation of L-α-glyceryl phosphorylcholine by hydrolysis of soy lecithin using phospholipase A1 in a novel solvent-free water in oil system
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DOI：
10.1016/j.lwt.2020.109562
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发表时间：
2020-07-01
影响因子：
6
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Cai, Zi-zhe;Wang, Hong-fei;Wang, Yong
With various applications in the food, pharmaceutical and cosmetics industries, L-Alpha-glycerylphosphorylcholine (L-alpha-GPC), is a valuable cholinergic substance whose natural resources is limited. Reported enzymatic pathway seems promising comparing chemical synthesis, while the low solubility of PC in water hinders its scaling-up production and involvement of organic solvent entails potential risks. A novel water-in-soybean oil solvent-free medium was applied in enzymatic preparation of L-alpha-GPC catalyzed by phospholipase A(1), where 99.1% of the PC in soy lecithin was hydrolyzed with L-alpha-GPC yield of 95.8% (conditions: oil to PC ratio 4:1, water addition 30 wt%, enzyme loading 5 wt%, 55 degrees C, 3 h). The purity and recovery of purified (silica chromatography) L-alpha-GPC a with 94.8% and 83.0%, respectively. Efficient and intensive L-alpha-GPC preparation could be achieved using soybean oil medium, and the proposed system is with advantages of solvent-free, significant reduction of water, offering a green and practical approach for future L-alpha-GPC production.
