A METHOD FOR FIXATION OF PREVIOUSLY FRESH-FROZEN HUMAN ADULT AND FETAL BRAINS THAT PRESERVES HISTOLOGICAL QUALITY AND IMMUNOREACTIVITY
A METHOD FOR FIXATION OF PREVIOUSLY FRESH-FROZEN HUMAN ADULT AND FETAL BRAINS THAT PRESERVES HISTOLOGICAL QUALITY AND IMMUNOREACTIVITY
复制标题
DOI：
10.1016/0165-0270(92)90006-y
复制
发表时间：
1992-09-01
影响因子：
3
通讯作者：
TOURTELLOTTE, WW
中科院分区：
文献类型：
--
作者：
JONES, EG;HENDRY, SHC;TOURTELLOTTE, WW
描述了一种能够固定先前新鲜冷冻和储存的成人和胎儿人或动物脑的方法。该方法包括在受控的冷冻保护条件下解冻期间固定，并且与良好的组织学质量和许多神经抗原的酶活性和免疫反应性的保存相容。它为储存大量组织提供了相当大的优势，可以从这些组织中取出多个样品，并在固定和其他条件下进行处理，这些条件可以针对各种方法进行优化，如果在储存前将整个大脑固定在单一固定剂中，则其中许多方法可能不相容。
A method is described that enables fixation of previously fresh-frozen and stored adult and fetal human or animal brains. The method involves fixing during thawing under controlled, cryoprotected conditions and is compatible with good histological quality and the preservation of enzymatic activity and immunoreactivity of many neural antigens. It offers considerable advantages for the storage of large amounts of tissue from which multiple samples can be taken and processed under fixation and other conditions that can be optimized for a variety of methods, many of which may be incompatible if the whole brain is fixed in a single fixative prior to storage.