DLK2 regulates arbuscule hyphal branching during arbuscular mycorrhizal symbiosis
DLK2 regulates arbuscule hyphal branching during arbuscular mycorrhizal symbiosis
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DOI：
10.1111/nph.16938
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发表时间：
2020-10-20
期刊：
影响因子：
9.4
通讯作者：
Garcia-Garrido, Jose Manuel
中科院分区：
文献类型：
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作者：
Ho-Plagaro, Tania;Morcillo, Rafael J. L.;Garcia-Garrido, Jose Manuel
D14和KAI 2受体分别使植物能够区分独脚金内酯（SL）和卡里金（KARs），以触发适当的环境和发育反应。这两种受体都与丛枝菌根（AM）形成的调节有关，并且是α，β-水解酶的RsbQ样家族的成员。DLK 2蛋白，其功能仍然未知，构成了RsbQ样蛋白家族的第三个分支。我们研究了tomatoSLK 2是否是AM共生中的一个新的调节组分。通过遗传学方法分析SlDLK 2的表达，并了解SlDLK 2在AM共生中的功能。我们发现SlDLK 2在根中的表达是AM依赖的，并且与含有丛枝的细胞相关; SlDLK 2的异位表达阻止了丛枝分枝并下调了AM响应基因，即使在没有共生的情况下也是如此;而在SlDLK 2沉默的情况下观察到了相反的效果。此外，SLDLK 2在蒺藜苜蓿根中的过表达表现出与在番茄根中观察到的相同的改变的表型。有趣的是，SlDLK 2与DELLA相互作用，DELLA是一种调节AM根中丛枝形成/降解的蛋白质。我们认为SlDLK 2是AM共生中调节丛枝植物生命周期的复杂植物介导机制的新组成部分。
D14 and KAI2 receptors enable plants to distinguish between strigolactones (SLs) and karrikins (KARs), respectively, in order to trigger appropriate environmental and developmental responses. Both receptors are related to the regulation of arbuscular mycorrhiza (AM) formation and are members of the RsbQ-like family of alpha,beta-hydrolases. DLK2 proteins, whose function remains unknown, constitute a third clade from the RsbQ-like protein family. We investigated whether the tomatoSlDLK2is a new regulatory component in the AM symbiosis. Genetic approaches were conducted to analyzeSlDLK2expression and to understand SlDLK2 function in AM symbiosis. We show thatSlDLK2expression in roots is AM-dependent and is associated with cells containing arbuscules.SlDLK2ectopic expression arrests arbuscule branching and downregulates AM-responsive genes, even in the absence of symbiosis; while the opposite effect was observed uponSlDLK2silencing. Moreover,SlDLK2overexpression inMedicago truncatularoots showed the same altered phenotype observed in tomato roots. Interestingly, SlDLK2 interacts with DELLA, a protein that regulates arbuscule formation/degradation in AM roots. We propose that SlDLK2 is a new component of the complex plant-mediated mechanism regulating the life cycle of arbuscules in AM symbiosis.