Mayday sustains trans-synaptic BMP signaling required for synaptic maintenance with age.
Mayday sustains trans-synaptic BMP signaling required for synaptic maintenance with age.
复制标题
随着年龄的增长，"五月天 "能维持突触维持所需的跨突触 BMP 信号。
DOI：
10.7554/elife.54932
复制
发表时间：
2021-03-05
期刊：
影响因子：
7.7
通讯作者：
Babcock D
中科院分区：
文献类型：
--
作者：
Sidisky JM;Weaver D;Hussain S;Okumus M;Caratenuto R;Babcock D
长期维持突触结构和功能对整个神经系统的功能和生存至关重要。突触发育背后的过程被很好地理解了。然而，在生物体的整个生命周期中维持突触的机制尚不清楚。在这里，我们证明了一个以前未被表征的基因CG31475调节成年果蝇NMJs的突触维持。我们将CG31475命名为求救，是因为随着年龄的增长，飞行能力和突触结构逐渐丧失。Mayday在功能上与人类蛋白质Cab45相似，Cab45负责从Trans Golgi网络（TGN）中分类分泌货物。我们发现，为了维持正常的突触结构和功能，Mayday需要在成年NMJs中维持跨突触BMP信号。最后，我们发现mayday的突变导致突触前运动神经元和突触后肌肉的损失，强调了维持突触完整性对细胞活力的重要性。
Maintaining synaptic structure and function over time is vital for overall nervous system function and survival. The processes that underly synaptic development are well understood. However, the mechanisms responsible for sustaining synapses throughout the lifespan of an organism are poorly understood. Here, we demonstrate that a previously uncharacterized gene, CG31475, regulates synaptic maintenance in adult Drosophila NMJs. We named CG31475 mayday due to the progressive loss of flight ability and synapse architecture with age. Mayday is functionally homologous to the human protein Cab45, which sorts secretory cargo from the Trans Golgi Network (TGN). We find that Mayday is required to maintain trans-synaptic BMP signaling at adult NMJs in order to sustain proper synaptic structure and function. Finally, we show that mutations in mayday result in the loss of both presynaptic motor neurons as well as postsynaptic muscles, highlighting the importance of maintaining synaptic integrity for cell viability.