Efforts to make and apply humanized yeast.
Efforts to make and apply humanized yeast.
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DOI：
10.1093/bfgp/elv041
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发表时间：
2016-03
影响因子：
4
通讯作者：
Marcotte EM
中科院分区：
文献类型：
--
作者：
Laurent JM;Young JH;Kachroo AH;Marcotte EM
尽管经历了十亿年的不同进化，面包酵母酿酒酵母长期以来一直被证明是研究人类生物学的宝贵模式生物。鉴于其易于处理和易于遗传操作，沿着与人类的广泛遗传保守性，研究人员能够通过在酵母中表达人类蛋白质，或通过人源化特定的酵母氨基酸，蛋白质甚至整个途径来扩展其效用，这也许并不奇怪。这些方法的通量越来越大，进一步使得能够在简化的模型生物体中详细研究人类生物学和人类基因的疾病特异性变异。
Despite a billion years of divergent evolution, the baker’s yeast Saccharomyces cerevisiae has long proven to be an invaluable model organism for studying human biology. Given its tractability and ease of genetic manipulation, along with extensive genetic conservation with humans, it is perhaps no surprise that researchers have been able to expand its utility by expressing human proteins in yeast, or by humanizing specific yeast amino acids, proteins or even entire pathways. These methods are increasingly being scaled in throughput, further enabling the detailed investigation of human biology and disease-specific variations of human genes in a simplified model organism.