Ca2+ and pH modulate alternative splicing of exon 5 in NMDA receptor subunit 1.
Ca2+ and pH modulate alternative splicing of exon 5 in NMDA receptor subunit 1.
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DOI：
10.1097/00001756-199911260-00036
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发表时间：
1999-11
期刊：
影响因子：
1.7
通讯作者：
M. Vallano;C. Beaman-Hall;S. Benmansour
中科院分区：
文献类型：
--
作者：
M. Vallano;C. Beaman-Hall;S. Benmansour
RT-PCR and intracellular Ca2+ measurements were used to identify factors that modulate alternative splicing of exon 5 in the NMDA receptor transcript encoding NR1, in cultured cerebellar granule neurons. Although cells grown in media containing 5 mM KCl demonstrate compromised survival, they show the predicted developmental transition from NR1a (-exon 5) to NR1b (+exon 5) mRNA expression. This transition was blocked under culture conditions that promote survival; inclusion or exclusion of exon 5 is a reversible process that is sensitive to alterations in Ca2+ and pH. We conclude that alternative splicing of NR1 pre-mRNA transcripts may be regulated by developmental cues that modulate the degree of glutamate receptor activation.
