DEVELOPMENT OF A MINIMALLY DEFINED MEDIUM FOR THE ACETOGEN CLOSTRIDIUM-THERMOACETICUM
DEVELOPMENT OF A MINIMALLY DEFINED MEDIUM FOR THE ACETOGEN CLOSTRIDIUM-THERMOACETICUM
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DOI：
10.1128/jb.159.2.700-703.1984
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发表时间：
1984-01-01
影响因子：
3.2
通讯作者：
DRAKE, HL
中科院分区：
文献类型：
--
作者：
LUNDIE, LL;DRAKE, HL
开发了一种最低限度的产乙酸菌的培养基热醋酸杆菌。该培养基以葡萄糖为碳源和能源，(NH4)2SO4为氮源，烟酸为唯一必需维生素、还原剂、磷酸盐-碳酸氢盐缓冲液、无机盐和螯合剂以及CO2气相。将热醋酸杆菌从含有酵母膏和胰蛋白内酯的未定义的培养基转到最低定义的培养基时，需要在添加了氨基酸和维生素的限定的培养基上连续传代。在微量培养基上生长和细胞产量下降，但CO脱氢酶、氢酶和甲酸脱氢酶的活性在未定义的和最低限度的培养上是相似的。
A minimally defined medium was developed for the cultivation of the acetogen C. thermoaceticum. The medium contained glucose as the C and energy source, (NH4)2 SO4 as the N source, nicotinic acid as the sole essential vitamin, reductant, a phosphate-bicarbonate buffer, mineral salts and chelator and a CO2 gas phase. Adaptation of C. thermoaceticum from undefined medium containing yeast extract and tryptone to the minimally defined medium required sequential passage on defined medium supplemented with amino acids and vitamins. Growth and cell yields were reduced on the minimal medium, but the activities of CO dehydrogenase, hydrogenase and formate dehydrogenase were comparable between undefined and minimal media.