Implementation and Validation of an Automated Flow Cytometry Analysis Pipeline for Human Immune Profiling
Implementation and Validation of an Automated Flow Cytometry Analysis Pipeline for Human Immune Profiling
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DOI：
10.1002/cyto.a.23664
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发表时间：
2019-02-01
期刊：
影响因子：
3.7
通讯作者：
Redmond, William L.
中科院分区：
文献类型：
--
作者：
Conrad, Valerie K.;Dubay, Christopher J.;Redmond, William L.
自动化试剂制备、样品处理和数据采集提高了流式细胞术数据的生成速度。此外，技术和流式细胞仪仪器的进步不断增加该数据的复杂性和维度。总之，这导致手动数据分析的压力增加，而手动数据分析具有固有的局限性，包括分析师的主观性和数据处理所需的时间长度。这些问题可能会在数据处理工作流程中造成瓶颈，并可能损害数据质量。为了解决这些问题以及在大容量人体免疫分析实验室中与手动门控相关的挑战，我们寻求实施自动化分析流程。在本报告中，我们讨论了选择自动化分析方法的注意事项、实施自动化管道的过程，并详细介绍了我们将自动化门控策略与 flowDensity 成功结合到我们的分析工作流程中。这一经过验证的管道增强了我们实验室为参与癌症免疫治疗临床试验的患者提供快速高通量免疫分析的能力。 (c) 国际细胞计数促进会
Automated reagent preparation, sample processing, and data acquisition have increased the rate at which flow cytometry data can be generated. Furthermore, advances in technology and flow cytometry instrumentation continually increase the complexity and dimensionality of this data. Together, this leads to increased pressure on manual data analysis, which has inherent limitations including subjectivity of the analyst and the length of time needed for data processing. These issues can create bottlenecks in the data processing workflow and potentially compromise data quality. To address these issues, as well as the challenges associated with manual gating in a high-volume human immune profiling laboratory, we sought to implement an automated analysis pipeline. In this report, we discuss considerations for selecting an automated analysis method, the process of implementing an automated pipeline, and detail our successful incorporation of an automated gating strategy with flowDensity into our analysis workflow. This validated pipeline augments our laboratory's ability to provide rapid high-throughput immune profiling for patients participating in cancer immunotherapy clinical trials. (c) International Society for Advancement of Cytometry