Potential Applications of Thermophilic Bacteriophages in One Health.
Potential Applications of Thermophilic Bacteriophages in One Health.
复制标题
嗜热噬菌体在健康领域的潜在应用。
DOI：
10.3390/ijms24098222
复制
发表时间：
2023-05-04
影响因子：
5.6
通讯作者：
Tumban, Ebenezer
中科院分区：
文献类型：
--
作者：
Liu, Hong;Kheirvari, Milad;Tumban, Ebenezer
关键词：
噬菌体具有广泛的应用，例如对抗抗生素耐药性、防止食品污染以确保食品安全，以及作为间接评估水质的生物标志物。此外，噬菌体成分（内溶素和外壳蛋白）在食品加工、疫苗设计和向体内输送货物方面有很多应用。因此，噬菌体/成分在人类、植物/兽医和环境健康（One Health）方面具有多种应用。尽管噬菌体/成分用途广泛，但其使用大多限于 4–40 °C 的温度。这限制了它们的应用（例如，在食品加工条件、巴氏灭菌和疫苗设计中）。嗜热噬菌体研究的进展发现了可用于食品加工和兽医学的新型嗜热内溶素（例如 ΦGVE2 酰胺酶和 MMPphg）。细胞内溶素在 > 65 °C 的温度下具有热稳定性，并具有广泛的抗菌活性。除了嗜热内溶素之外，源自噬菌体的酶（DNA 聚合酶和连接酶）在分子生物学/生物技术中具有不同的应用：生成 DNA 文库并开发人类和动物病原体的诊断方法。此外，正在探索来自噬菌体的外壳蛋白，以开发在人类和动物健康中具有多种应用的病毒样颗粒平台。总体而言，噬菌体，尤其是嗜热噬菌体，在 One Health 中有大量应用。
Bacteriophages have a wide range of applications such as combating antibiotic resistance, preventing food contamination for food safety, and as biomarkers to indirectly assess the quality of water. Additionally, bacteriophage components (endolysins and coat proteins) have a lot of applications in food processing, vaccine design, and the delivery of cargo to the body. Therefore, bacteriophages/components have a multitude of applications in human, plant/veterinary, and environmental health (One Health). Despite their versatility, bacteriophage/component use is mostly limited to temperatures within 4–40 °C. This limits their applications (e.g., in food processing conditions, pasteurization, and vaccine design). Advances in thermophilic bacteriophage research have uncovered novel thermophilic endolysins (e.g., ΦGVE2 amidase and MMPphg) that can be used in food processing and in veterinary medicine. The endolysins are thermostable at temperatures > 65 °C and have broad antimicrobial activities. In addition to thermophilic endolysins, enzymes (DNA polymerase and ligases) derived from thermophages have different applications in molecular biology/biotechnology: to generate DNA libraries and develop diagnostics for human and animal pathogens. Furthermore, coat proteins from thermophages are being explored to develop virus-like particle platforms with versatile applications in human and animal health. Overall, bacteriophages, especially those that are thermophilic, have a plethora of applications in One Health.
登录
查看更多内容
影响因子：
5.1
作者：
Edstam, JS;Dulmaa, N;Densmaa, B
通讯作者：
Densmaa, B
影响因子：
17.1
作者：
Ashley CE;Carnes EC;Phillips GK;Durfee PN;Buley MD;Lino CA;Padilla DP;Phillips B;Carter MB;Willman CL;Brinker CJ;Caldeira Jdo C;Chackerian B;Wharton W;Peabody DS
通讯作者：
Peabody DS
影响因子：
5.5
作者：
Basu, Rupsa;Zhai, Lukai;Tumban, Ebenezer
通讯作者：
Tumban, Ebenezer
影响因子：
3.7
作者：
Skowron PM;Kropinski AM;Zebrowska J;Janus L;Szemiako K;Czajkowska E;Maciejewska N;Skowron M;Łoś J;Łoś M;Zylicz-Stachula A
通讯作者：
Zylicz-Stachula A
影响因子：
5.2
作者：
Chander Y;Koelbl J;Puckett J;Moser MJ;Klingele AJ;Liles MR;Carrias A;Mead DA;Schoenfeld TW
通讯作者：
Schoenfeld TW