Preparation, characterization and long-term antibacterial activity of Ag–poly(dopamine)–TiO2 nanotube composites
Preparation, characterization and long-term antibacterial activity of Ag–poly(dopamine)–TiO2 nanotube composites
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DOI：
10.1039/c5ra22061k
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发表时间：
2016-02
期刊：
影响因子：
3.9
通讯作者：
Hongfen Wang;Luyao Wei;Zhiqi Wang;Shougang Chen
中科院分区：
文献类型：
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作者：
Hongfen Wang;Luyao Wei;Zhiqi Wang;Shougang Chen
采用一种简单有效的方法将银纳米粒子负载到聚多巴胺修饰的二氧化钛纳米管（PDA-TNTs）上，制备了银纳米粒子-聚多巴胺-二氧化钛纳米管复合物（Ag-PDA-TNTs），并将其作为一种长效抗菌剂应用于抑制细菌细胞的生长。采用TEM、XRD、FT-IR和XPS等分析手段对所制备的Ag-PDA-TNTs进行了表征。将锐钛矿TNT包封在PDA层中，厚度为约3 nm，约20.4wt%的单晶Ag纳米颗粒锚定在PDA层上。XPS和FT-IR结果表明，PDA层不仅作为还原剂，可以还原Ag+离子的Ag纳米粒子，但也作为粘合剂涂层通过拴系的Ag纳米粒子的PDA-TNT的表面。纳米复合材料的长期释放曲线表明，PDA层减缓了银纳米粒子的释放速率，这意味着可能的长期抗菌活性的Ag-PDA-TNTs;和抗菌试验验证了这一点。此外，Ag-PDA-TNTs在可见光下的抗菌活性高于在黑暗中的抗菌活性，这是由于可见光下Ag纳米粒子与活性氧协同抗菌的结果。与载银纳米TiO 2纳米管（Ag-TNTs）相比，Ag-PDA-TNTs具有更高的抗菌活性和更长的抗菌时间，这是由于PDA层对纳米Ag的束缚作用和Ag+离子释放速率的减缓。本工作为银系抗菌剂的制备提供了一种新的方法，有利于银系抗菌剂在现代医药和生物医学领域的实际应用。
A simple and efficient approach for the loading of Ag nanoparticles on poly(dopamine)-modified TiO2 nanotubes (PDA–TNTs) was used to prepare a Ag nanoparticle–poly(dopamine)–TiO2 nanotube composite (Ag–PDA–TNTs) that was applied as a long-term antibacterial agent to inhibit the growth of bacterial cells. The features of the obtained Ag–PDA–TNTs were investigated by TEM, XRD, FT-IR and XPS analysis. Anatase TNTs were encapsulated in PDA layers with a thickness of about 3 nm on which about 20.4 wt% of single crystalline Ag nanoparticles anchored. XPS and FT-IR results indicated that the PDA layer not only served as a reduction reagent that could reduce Ag+ ions to Ag nanoparticles but also worked as an adhesive coating by tethering the Ag nanoparticles on the surface of PDA–TNTs. The long-term release profiles of the nanocomposites showed that PDA layers slowed the release rate of Ag nanoparticles, implying the possible long-term antibacterial activity of Ag–PDA–TNTs; and the antibacterial assays verified this point. Besides that, the antibacterial activity of Ag–PDA–TNTs under visible light was higher than that in the dark because of the synergistically antibacterial effects of Ag nanoparticles and ROS under visible light irradiation. Compared with the antibacterial activity of TiO2 nanotubes loaded with Ag nanoparticles (Ag–TNTs), Ag–PDA–TNTs had higher and longer-term antibacterial activity which is attributed to the effect of PDA layers tethering Ag nanoparticles on TNTs and slowing Ag+ ions release rate. This work provides a new method for the preparation of Ag-based antibacterial agents and facilitates their practical application in modern antifouling and biomedical fields.