Nuclear transfer in cats and its application
Nuclear transfer in cats and its application
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DOI：
10.1016/j.theriogenology.2006.03.017
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发表时间：
2006-07-01
期刊：
影响因子：
2.8
通讯作者：
Dresser, B. L.
中科院分区：
文献类型：
--
作者：
Gomez, M. C.;Pope, C. E.;Dresser, B. L.
核移植（NT）技术通常用于产生相同的个体，但它也是了解核重编程的细胞和分子方面的强大资源。最近，该程序已用于人类生产患者特异性胚胎干细胞。NT在猫中的成功应用通过家养和非家养克隆小猫的出生证明，其效率与其他哺乳动物物种的报道相似。在猫中，已经证明体内或体外成熟的卵母细胞可以用作供体胞质。卵母细胞体外成熟的时间长短影响重构胚胎的体外发育，体外成熟时间较短的卵母细胞是供体胞质的首选来源。对于NT，可以通过使用不同的细胞同步化方法将猫体细胞同步化到细胞周期的G 0/G1期，而不影响克隆胚胎体外发育的频率。此外，胚胎在体外发育到胚泡阶段不受细胞类型的影响，但细胞类型对正常后代的影响需要评估。种间NT具有潜在的应用保护濒危猫科动物，作为活的后代的男性和女性的非洲野猫（AWC，Felis silvestris lybica）已经出生和怀孕后，转移黑脚猫（Felis nigripes）克隆胚胎到家猫（Felis silvestris卡图斯）受体产生。此外，成功地在体外胚胎发育到囊胚阶段后，非家养猫科动物的体细胞进入家猫卵母细胞的属间NT已经实现，但没有获得可行的后代。因此，虽然猫的细胞质诱导早期核重塑的细胞核从不同的属，早期胚胎发育停滞的高发病率可能是由异常的核重编程。将AWC克隆胚胎移植给家猫受体后，在妊娠的各个阶段经常观察到胚胎吸收和流产。腹腔脏器外置、呼吸衰竭、败血症等是克隆幼猫死亡的主要原因。尽管如此，几只活的家养和AWC克隆小猫已经出生，它们看起来正常健康。重要的是要在这些动物的一生中继续评估它们，并检查它们的自然繁殖能力。(c)2006年爱思唯尔公司All rights reserved.
Nuclear transfer (NT) technology is typically used for generating identical individuals, but it is also a powerful resource for understanding the cellular and molecular aspects of nuclear reprogramming. Most recently, the procedure has been used in humans for producing patient-specific embryonic stem cells. The successful application of NT in cats was demonstrated by the birth of domestic and non-domestic cloned kittens at a similar level of efficiency to that reported for other mammalian species. In cats, it has been demonstrated that either in vivo or in vitro matured oocytes can be used as donor cytoplasts. The length of in vitro oocyte maturation affects in vitro development of reconstructed embryos, and oocytes matured in vitro for shorter periods of time are the preferred source of donor cytoplasts. For NT, cat somatic cells can be synchronized into the G0/G1 phase of the cell cycle by using different methods of cell synchronization without affecting the frequency of in vitro development of cloned embryos. Also, embryo development to the blastocyst stage in vitro is not influenced by cell type, but the effect of cell type on the percentage of normal offspring produced requires evaluation. Inter-species NT has potential application for preserving endangered felids, as live offspring of male and female African wildcats (AWC, Felis silvestris lybica) have been born and pregnancies have been produced after transferring black-footed cat (Felis nigripes) cloned embryos into domestic cat (Felis silvestris catus) recipients. Also, successful in vitro embryo development to the blastocyst stage has been achieved after inter-generic NT of somatic cells of non-domestic felids into domestic cat oocytes, but no viable progeny have been obtained. Thus, while cat cytoplasm induces early nuclear remodeling of cell nuclei from a different genus, the high incidence of early embryo developmental arrest may be caused by abnormal nuclear reprogramming. Fetal resorption and abortions were frequently observed at various stages of pregnancy after transfer of AWC cloned embryos into domestic cat recipients. Abnormalities, such as abdominal organ exteriorization and respiratory failure and septicemia were the main causes of death in neonatal cloned kittens. Nonetheless, several live domestic and AWC cloned kittens have been born that are seemingly normal and healthy. It is important to continue evaluating these animals throughout their lives and to examine their capability for natural reproduction. (c) 2006 Elsevier Inc. All rights reserved.