Comparative Analysis of two Component Signal Transduction Systems of the Lactobacillus Acidophilus Group.
Comparative Analysis of two Component Signal Transduction Systems of the Lactobacillus Acidophilus Group.
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DOI：
10.1590/s1517-83822011000100019
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发表时间：
2011-01
期刊：
影响因子：
--
通讯作者：
Qu X
中科院分区：
文献类型：
--
作者：
Cui Y;Qu X
嗜酸乳杆菌群是密切相关的乳酸杆菌的遗传学上不同的群。这一群体的成员被认为具有益生菌特性，并占据不同的环境生态位。细菌通常通过由组氨酸蛋白激酶（HPK）及其同源反应调节因子（RR）组成的双组分系统（TCS）来感知和响应环境变化。利用计算机技术，这五个完全测序的L。扫描嗜酸乳杆菌组基因组以预测TCS。在该菌的单个基因组中检测到5 ~ 9个推定的TCS编码基因。嗜酸菌群。洛杉矶使用Grebe和Stock分类法将嗜酸乳杆菌群HPK和RR分为亚科。对推定的TCS进行保守结构域分析以预测生物学功能。预测了L.嗜酸乳杆菌HPKs和RRs的活性与其它微生物的活性进行了比较。一些TCS参与了与益生菌能力相关的多种功能，包括对酸和胆汁的耐受性、抗菌肽的产生、对糖肽抗生素万古霉素的耐受性以及氧化条件。
The Lactobacillus acidophilus group is a phylogenetically distinct group of closely related lactobacilli. Members of this group are considered to have probiotic properties and occupy different environmental niches. Bacteria generally sense and respond to environmental changes through two component systems (TCSs) which consist of a histidine protein kinase (HPK) and its cognate response regulator (RR). With the use of in silico techniques, the five completely sequenced L. acidophilus group genomes were scanned in order to predict TCSs. Five to nine putative TCSs encoding genes were detected in individual genomes of the L. acidophilus group. The L. acidophilus group HPKs and RRs were classified into subfamilies using the Grebe and Stock classification method. Putative TCSs were analyzed with respect to conserved domains to predict biological functions. Putative biological functions were predicted for the L. acidophilus group HPKs and RRs by comparing them with those of other microorganisms. Some of TCSs were putatively involved in a wide variety of functions which are related with probiotic ability, including tolerance to acid and bile, production of antimicrobial peptides, resistibility to the glycopeptide antibiotic vancomycin, and oxidative condition.