Endocardium Contributes to Cardiac Fat.
Endocardium Contributes to Cardiac Fat.
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DOI：
10.1161/circresaha.115.307202
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发表时间：
2016-01
影响因子：
20.1
通讯作者：
Hui Zhang;Wenjuan Pu;Qiaozhen Liu;Lingjuan He;Xiuzhen Huang;Xueying Tian;Libo Zhang;Y. Nie;Shengshou Hu;Kathy O. Lui;Bin Zhou
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文献类型：
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作者：
Hui Zhang;Wenjuan Pu;Qiaozhen Liu;Lingjuan He;Xiuzhen Huang;Xueying Tian;Libo Zhang;Y. Nie;Shengshou Hu;Kathy O. Lui;Bin Zhou
阐明心脏脂肪的发育起源可以为心血管疾病的治疗提供重要的意义。最近对心外膜脂肪组织的间皮来源的鉴定揭示了成人心脏中脂肪细胞的异质起源。然而，心脏内脂肪细胞的发育起源，即心肌内脂肪细胞，在很大程度上仍然未知。目的探讨心肌内脂肪细胞的发育来源。方法和结果在这项研究中，我们发现大多数心肌内脂肪细胞局限于靠近内膜的心肌区域。利用内皮细胞的遗传谱系追踪模型，我们发现Nfatc 1（+）内皮细胞参与了大量的心肌内脂肪细胞。尽管内皮细胞有能力在心肌内脂肪细胞周围产生冠状血管内皮细胞，但我们的谱系追踪分析结果表明，心肌内脂肪细胞并非来源于冠状血管。然而，出生后心脏的内皮细胞在体内平衡期间或心肌梗死后对心肌内脂肪细胞没有贡献。结论：我们的体内脂肪分布研究表明，在成人心脏中，发育中的内皮细胞而不是血管内皮细胞产生心肌内脂肪细胞。
RATIONALE Unraveling the developmental origin of cardiac fat could offer important implications for the treatment of cardiovascular disease. The recent identification of the mesothelial source of epicardial fat tissues reveals a heterogeneous origin of adipocytes in the adult heart. However, the developmental origin of adipocytes inside the heart, namely intramyocardial adipocytes, remains largely unknown. OBJECTIVE To trace the developmental origin of intramyocardial adipocytes. METHODS AND RESULTS In this study, we identified that the majority of intramyocardial adipocytes were restricted to myocardial regions in close proximity to the endocardium. Using a genetic lineage tracing model of endocardial cells, we found that Nfatc1(+) endocardial cells contributed to a substantial number of intramyocardial adipocytes. Despite the capability of the endocardium to generate coronary vascular endothelial cells surrounding the intramyocardial adipocytes, results from our lineage tracing analyses showed that intramyocardial adipocytes were not derived from coronary vessels. Nevertheless, the endocardium of the postnatal heart did not contribute to intramyocardial adipocytes during homeostasis or after myocardial infarction. CONCLUSIONS Our in vivo fate-mapping studies demonstrated that the developing endocardium, but not the vascular endothelial cells, gives rise to intramyocardial adipocytes in the adult heart.