Albumin Microvascular Leakage in Brains with Diabetes Mellitus
Albumin Microvascular Leakage in Brains with Diabetes Mellitus
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DOI：
10.1002/jemt.22708
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发表时间：
2016-09-01
影响因子：
2.5
通讯作者：
Ueno, Masaki
中科院分区：
文献类型：
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作者：
Fujihara, Ryuji;Chiba, Yoichi;Ueno, Masaki
他们的目的是研究糖尿病db/db小鼠脑室周围区域内源性白蛋白（血脑屏障（BBB）损伤的代表性标志）的微血管渗漏是否由于老年糖尿病患者脑室周围白质高强度的磁共振成像形成加速而引起。采用光镜、电镜和半定量分析技术，检测了db/db小鼠和db/+m对照小鼠脑室周围区和脊髓内源性白蛋白的免疫反应性，表明血管通透性。与对照组相比，db/db小鼠心室周围血管壁白蛋白的免疫反应性更强。此外，在db/db小鼠和对照组的脊髓中观察到弱的免疫反应性。表明白蛋白免疫反应性的金颗粒在db/db小鼠血管周围区域的数量显著高于对照组，但在脊髓内的颗粒数量与对照组无显著差异。这些发现表明，白蛋白微血管渗漏，或血脑屏障破坏，是诱导糖尿病小鼠心室周围区域。Microsc。科学通报，2016,39(3):833-837。(C) 2016 Wiley期刊公司
Their aim was to examine whether microvascular leakage of endogenous albumin, a representative marker for blood-brain barrier (BBB) damage, was induced in the periventricular area of diabetic db/db mice because periventricular white matter hyperintensity formation in magnetic resonance images was accelerating in elderly patients with diabetes mellitus. Using light and electron microscopes, and semi-quantitative analysis techniques, immunoreactivity of endogenous albumin, indicating vascular permeability, was examined in the periventricular area and spinal cord of db/db mice and db/+m control mice. Greater immunoreactivity of albumin was observed in the vessel wall of the periventricular area of db/db mice than in controls. Additionally, weak immunoreactivity was observed in the spinal cord of both db/db mice and controls. The number of gold particles, indicating immunoreactivity of albumin, in the perivascular area of db/db mice was significantly higher than that of control mice, but there was no significant difference in the number of particles in the spinal cord between db/db mice and controls. These findings suggest that albumin microvascular leakage, or BBB breakdown, is induced in the periventricular area of diabetic mice. Microsc. Res. Tech. 79:833-837, 2016. (C) 2016 Wiley Periodicals, Inc.