SELF-COMPLEMENTARY OLIGOPEPTIDE MATRICES SUPPORT MAMMALIAN-CELL ATTACHMENT
SELF-COMPLEMENTARY OLIGOPEPTIDE MATRICES SUPPORT MAMMALIAN-CELL ATTACHMENT
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DOI：
10.1016/0142-9612(95)96874-y
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发表时间：
1995-12-01
期刊：
影响因子：
14
通讯作者：
RICH, A
中科院分区：
文献类型：
--
作者：
ZHANG, SG;HOLMES, TC;RICH, A
描述了一类新的离子型自互补寡肽，其中两个成员已被指定为RAD 16和EAK 16。这些寡肽由交替的离子亲水性和疏水性氨基酸的规则重复组成，并在水中缔合形成稳定的β-折叠结构。添加含有毫摩尔量的单价盐的缓冲液或将肽溶液转移到生理溶液中导致寡肽自发组装成稳定的宏观膜基质。基质由有序的细丝组成，这些细丝形成多孔外壳。多种哺乳动物细胞类型能够附着于RAD 16和EAK 16膜基质。这些基质提供了一种新的实验系统，用于分析体外细胞附着的机制，并可能在组织再生，组织移植和伤口愈合的体内研究中有应用。
A new class of ionic self-complementary oligopeptides is described, two members of which have been designated RAD16 and EAK16. These oligopeptides consist of regular repeats of alternating ionic hydrophilic and hydrophobic amino acids and associate to form stable beta-sheet structures in water. The addition of buffers containing millimolar amounts of monovalent salts or the transfer of a peptide solution into physiological solutions results in the spontaneous assembly of the oligopeptides into a stable, macroscopic membranous matrix. The matrix is composed of ordered filaments which form porous enclosures. A variety of mammalian cell types are able to attach to both RAD16 and EAK16 membranous matrices. These matrices provide a novel experimental system for analysing mechanisms of in vitro cell attachment and may have applications in in vivo studies of tissue regeneration, tissue transplantation and wound healing.