The Thylakoid Membrane Proteome of Two Marine Diatoms Outlines Both Diatom-Specific and Species-Specific Features of the Photosynthetic Machinery
The Thylakoid Membrane Proteome of Two Marine Diatoms Outlines Both Diatom-Specific and Species-Specific Features of the Photosynthetic Machinery
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DOI：
10.1021/pr200600f
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发表时间：
2011-12-01
影响因子：
4.4
通讯作者：
Aro, Eva-Mari
中科院分区：
文献类型：
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作者：
Grouneva, Irina;Rokka, Anne;Aro, Eva-Mari
光合自养生物的类囊体膜含有光合电子传递链的主要成分。通过二维蓝色原生 (BN)/SDS-PAGE 结合质谱分析，创建了两种海洋硅藻（假微型海链藻和三角褐指藻）的类囊体蛋白复合物的详细蛋白质组图谱。鉴定出一种新型硅藻特异性光系统 I (PS I) 相关蛋白。第二种可能具有 PS I 相互作用的质体靶向蛋白被发现仅限于中心硅藻物种 T.pseudonana。 PGR5/PGRL 同源物被发现是两个物种共有的 PS I 介导的循环电子传递的唯一蛋白质成分。首次提出了类似 LI818 的光捕获蛋白 (Lhcx) 可能位于 PSI 的证据。这项研究还推进了目前对光捕获天线组成和 T.pseudonana 中 Lhcx 在蛋白质水平上表达的认识，并详细介绍了 Lhcx 在硅藻中的分子分布。上述蛋白质和其他几种功能未知的蛋白质为进一步的诱变分析提供了广泛的基础，旨在进一步了解硅藻光合机构的组成和功能。这项研究的蛋白质组学方法进一步充当了确认和改进基因组衍生蛋白质模型的工具。
The thylakoid membrane of photoautotrophic organisms contains the main components of the photosynthetic electron transport chain. Detailed proteome maps of the thylakoid protein complexes of two marine diatoms, Thalassiosira pseudonana and Phaeodactylum tricornutum, were created by means of two-dimensional blue native (BN)/SDS-PAGE coupled with mass spectrometry analysis. One novel diatomspecific photosystem I (PS I)-associated protein was identified. A second plastid-targeted protein with possible PS I interaction was discovered to be restricted to the centric diatom species T. pseudonana. PGR5/PGRL homologues were found to be the only protein components of PS I-mediated cyclic electron transport common to both species. For the first time, evidence for a possible PSI localization of LI818-like light harvesting proteins (Lhcx) is presented. This study also advances the current knowledge on the light harvesting antenna composition and Lhcx expression in T. pseudonana on the protein level and presents details on the molecular distribution of Lhcx in diatoms. Above mentioned proteins and several others with unknown function provide a broad basis for further mutagenesis analysis, aiming toward further understanding of the composition and function of the photosynthetic apparatus of diatoms. The proteomics approach of this study further served as a tool to confirm and improve genome-derived protein models.