Droplet electric separator microfluidic device for cell sorting
Droplet electric separator microfluidic device for cell sorting
复制标题
用于细胞分选的液滴电分离微流装置
DOI：
10.1063/1.3360812
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发表时间：
2010-05-10
影响因子：
4
通讯作者：
Zhao, Xing-Zhong
中科院分区：
文献类型：
--
作者：
Guo, Feng;Ji, Xing-Hu;Zhao, Xing-Zhong
开发了一种简单有效的用于细胞分选的液滴电分离微流装置。通过在通道旁的电极上施加直流电压，使未进行预充电操作的水滴在通道内沿电场方向移动一段距离，使目标液滴流向收集器。在所需的脉冲下，可以使用微电极以〜100 Hz的分选速率分离单个液滴。可以分别分选出封装在不含表面活性剂的藻酸盐液滴中的单个或多个哺乳动物细胞（HePG2）。该方法可用于单细胞操作或分析。
A simple and effective droplet electric separator microfluidic device was developed for cell sorting. The aqueous droplet without precharging operation was influenced to move a distance in the channel along the electric field direction by applying dc voltage on the electrodes beside the channel, which made the target droplet flowing to the collector. Single droplet can be isolated in a sorting rate of ∼100 Hz with microelectrodes under a required pulse. Single or multiple mammalian cell (HePG2) encapsulated in the surfactant free alginate droplet could be sorted out respectively. This method may be used for single cell operation or analysis.