Effect of Adipose-Derived Stem Cells and Their Exo as Adjunctive Therapy to Nonsurgical Periodontal Treatment: A Histologic and Histomorphometric Study in Rats
Effect of Adipose-Derived Stem Cells and Their Exo as Adjunctive Therapy to Nonsurgical Periodontal Treatment: A Histologic and Histomorphometric Study in Rats
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DOI：
10.3390/biom8040167
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发表时间：
2018-12-01
期刊：
影响因子：
5.5
通讯作者：
Sabry, Dina
中科院分区：
文献类型：
--
作者：
Mohammed, Ebtehal;Khalil, Eman;Sabry, Dina
在许多情况下，洁治和根面平整（SRP）的价值有限，因此采用了辅助治疗来提高其疗效。对脂肪来源的干/基质细胞（ADSCs）在牙周再生中的应用进行了研究，结果很有前景。然而，它们存在安全隐患。外泌体（Exo.）是介导干/基质细胞作用的细胞外囊泡，是克服这些隐患的一种新方法。在50只大鼠身上诱导结扎性牙周炎，持续14天，然后将它们分为对照组（5只健康大鼠用于组织学比较）、SRP组、ADSCs组和Exo.组，在第2天、第2周和第4周进行评估，每组在每个评估时间点各有5只大鼠。对标本进行组织学描述（H&E染色）、组织化学研究（Masson三色染色）和组织形态计量学研究，以评估新生组织的面积百分比。Exo.组在所有评估时间点都显示出最佳效果，新生组织面积百分比显著更高，其次是ADSCs组，最后是SRP组。Exo.组和ADSCs组都显示出有组织的新生组织，到第4周时，Exo.组获得了与正常健康组织相当的组织学结果。脂肪来源的干/基质细胞及其外泌体是一种对SRP有前景的辅助治疗方法。
Scaling and root planing (SRP) is of limited value in many cases, so adjunctive treatment was applied to augment its outcome. Adipose-derived stem/stromal cells (ADSCs) were investigated in periodontal regeneration with promising results. However, they have safety concerns. The exosomes (Exo.), which are extracellular vesicles mediating the action of stem/stromal cells, represent a new approach to overcome these concerns. Ligature-induced periodontitis was induced in 50 rats for 14 days, and they were divided into control (5 healthy rats for histologic comparison), SRP group, ADSCs group, and Exo. group, with evaluation intervals at 2 days, and 2 and 4 weeks, including 5 rats in each interval for each group. The specimens were evaluated for histologic description (H&E), histochemical study (Masson trichrome), and histomorphometric study, to evaluate the area % of newly formed tissues. The Exo. group revealed the best results in all intervals with significantly higher area % of newly formed tissues, followed by ADSCs and, finally, SRP. Both Exo. and ADSCs showed organized newly formed tissues with the Exo. group obtaining comparable histologic results to the normal, healthy tissues by 4 weeks. Adipose-derived stem/stromal cells and their Exo. represent a promising adjunctive treatment to SRP.