Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor
Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor
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DOI：
10.1007/s00253-002-1219-x
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发表时间：
2003-06-01
影响因子：
5
通讯作者：
Schüler, D
中科院分区：
文献类型：
--
作者：
Heyen, U;Schüler, D
培养基和生长条件进行了优化的微氧培养的Magnetocellum gryphiswaldense在烧瓶和发酵罐中，导致细胞和磁小体产量显着增加，与早期的研究相比。建立了一种自动控制发酵罐（oxystat）中低溶解氧张力（pO（2））的可靠方法。生长和磁小体的形成。gryphiswaldense，M.在不同的氧浓度下研究了magnetotacticum和Magnetotacticum sp. AMB-1。尽管它们对氧气的生长反应存在差异，但我们发现所有三种磁螺菌菌株中pO（2）与磁小体形成之间存在明显的相关性。磁铁矿生物矿化仅在低于20 mbar O-2的阈值下诱导，磁小体形成的最佳条件为pO（2）为0.25 mbar（1 bar = 10（5）Pa）。用M. Gryphiswaldense在Oxystat条件下生长，这是迄今为止报道的趋磁细菌的最高磁小体生产率。研究结果为在一定条件下大规模培养磁囊藻提供了依据。
Media and growth conditions were optimized for the microaerobic cultivation of Magnetospirillum gryphiswaldense in flasks and in a fermentor, resulting in significantly increased cell and magnetosome yields, compared with earlier studies. A reliable method was established for the automatic control of low dissolved oxygen tensions (pO(2)) in the fermentor (oxystat). Growth and magnetosome formation by M. gryphiswaldense, M. magnetotacticum and Magnetospirillum sp. AMB-1 were studied at various oxygen concentrations. Despite differences in their growth responses with respect to oxygen, we found a clear correlation between pO(2) and magnetosome formation in all three Magnetospirillum strains. Magnetite biomineralization was induced only below a threshold value of 20 mbar O-2 and optimum conditions for magnetosome formation were found at a pO(2) Of 0.25 mbar (1 bar = 10(5) Pa). A maximum yield of 6.3 mg magnetite l(-1) day(-1) was obtained with M. gryphiswaldense grown under oxystat conditions, which is the highest magnetosome productivity reported so far for a magnetotactic bacterium. In conclusion, the presented results provide the basis for large-scale cultivation of magnetospirilla under defined conditions.