The 14-amino acid CLV3, CLE19, and CLE40 peptides trigger consumption of the root meristem in Arabidopsis through a CLAVATA2-dependent pathway
The 14-amino acid CLV3, CLE19, and CLE40 peptides trigger consumption of the root meristem in Arabidopsis through a CLAVATA2-dependent pathway
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DOI：
10.1105/tpc.105.034009
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发表时间：
2005-09-01
期刊：
影响因子：
11.6
通讯作者：
Liu, CM
中科院分区：
文献类型：
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作者：
Fiers, M;Golemiec, E;Liu, CM
CLAVATA3 (CLV3)、CLV3/ESR19 (CLE19) 和 CLE40 属于拟南芥中 26 个基因的家族，这些基因编码未知身份的推定肽配体。先前已经表明，这三个基因中任何一个的异位表达都会导致根分生组织的消耗。在这里，我们表明，与保守的 CLE 基序相对应的合成 14 个氨基酸肽 CLV3p、CLE19p 和 CLE40p 的体外应用模拟了过度表达表型。当CLE19蛋白外用时观察到相同的结果。有趣的是，clv2 未能对肽治疗做出反应，这表明 CLV2 参与了 CLE 肽信号传导。 CLE19 过表达系与 clv 突变体的杂交证实了 CLV2 的参与。使用组织特异性标记线的分析表明，肽处理导致底层组织子细胞过早分化以及中柱鞘和内皮层细胞身份的错误指定。我们认为这些 14 个氨基酸肽代表了相应 CLE 蛋白的主要活性结构域，它们与根中未知的细胞身份维持 CLV2 受体复合物相互作用或使其饱和，导致根分生组织的消耗。
CLAVATA3 (CLV3), CLV3/ESR19 (CLE19), and CLE40 belong to a family of 26 genes in Arabidopsis thaliana that encode putative peptide ligands with unknown identity. It has been shown previously that ectopic expression of any of these three genes leads to a consumption of the root meristem. Here, we show that in vitro application of synthetic 14-amino acid peptides, CLV3p, CLE19p, and CLE40p, corresponding to the conserved CLE motif, mimics the overexpression phenotype. The same result was observed when CLE19 protein was applied externally. Interestingly, clv2 failed to respond to the peptide treatment, suggesting that CLV2 is involved in the CLE peptide signaling. Crossing of the CLE19 overexpression line with clv mutants confirms the involvement of CLV2. Analyses using tissue-specific marker lines revealed that the peptide treatments led to a premature differentiation of the ground tissue daughter cells and misspecification of cell identity in the pericycle and endodermis layers. We propose that these 14-amino acid peptides represent the major active domain of the corresponding CLE proteins, which interact with or saturate an unknown cell identity-maintaining CLV2 receptor complex in roots, leading to consumption of the root meristem.