GENE AND PROTEIN-SEQUENCE OF AN INFLUENZA NEURAMINIDASE WITH HEMAGGLUTININ ACTIVITY
GENE AND PROTEIN-SEQUENCE OF AN INFLUENZA NEURAMINIDASE WITH HEMAGGLUTININ ACTIVITY
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DOI：
10.1016/0042-6822(85)90206-5
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发表时间：
1985-01-01
期刊：
影响因子：
3.7
通讯作者：
COLMAN, PM
中科院分区：
文献类型：
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作者：
AIR, GM;RITCHIE, LR;COLMAN, PM
N9亚型流感病毒神经氨酸酶（NA）也具有血凝素（HA）活性。为了确定该NA与其他已知NA和HA亚型序列之间的序列关系，并作为确定完整结构的必要步骤，使用Sa/I连接子将流感病毒a/ tern/Australia/G70C/75的N9 NA编码序列的全长拷贝克隆到质粒pUC9中。通过定向亚克隆到单链噬菌体载体M13mp19和M13mp18上，并使用双脱氧程序对该基因进行测序。大部分NA序列也可通过对色氨酸的直接蛋白测序获得。与N1和N2序列相比，N9 NA的同源性分别为43%和44%。与任何已知的HA序列或副粘病毒SV5的血凝素-神经氨酸酶蛋白没有显著的同源性。与其他NA分子一样，N9 NA通过一个n端疏水区域锚定在膜上，生物活性头可以通过pronase从该区域释放。
An influenza virus neuraminidase (NA) of the N9 subtype also has hemagglutinin (HA) activity. To determine sequence relationships between this NA and other known NA and HA subtype sequences, and as a necessary step toward a complete structure determination, a full-length copy of the coding sequence of the N9 NA of influenza virus A/tern/Australia/G70C/75 was cloned into the plasmid pUC9 using Sa/I linkers. The gene was sequenced by directed subcloning into the single-stranded phage vectors M13mp19 and M13mp18 and use of the dideoxy procedure. Most of the NA sequence was also obtained by direct protein sequencing of tryptic peptides. The N9 NA has 43 and 44% homology when compared to N1 or N2 sequences, respectively. There is no significant homology to any known HA sequence, or to the hemagglutinin-neuraminidase protein of the paramyxovirus SV5. Like the other NA molecules, the N9 NA is anchored in the membrane by an N-terminal hydrophobic region, from which biologically active heads can be released by pronase.