Rox3 and Rts1 function in the global stress response pathway in baker's yeast.
Rox3 and Rts1 function in the global stress response pathway in baker's yeast.
复制标题
Rox3 和 Rts1 在面包酵母的整体应激反应途径中发挥作用。
DOI：
10.1093/genetics/142.4.1083
复制
发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Zitomer,RS
中科院分区：
文献类型：
--
作者：
EvangelistaJr,CC;RodriguezTorres,AM;Limbach,MP;Zitomer,RS
酵母通过一系列信号转导途径和膜上作用的STRE DNA序列介导的全局性应激反应来响应多种应激。TheCYC 7基因，编码异2-细胞色素c，已被证明响应热休克，葡萄糖饥饿，接近稳定期，并在这里，我们证明，渗透胁迫。这种反应在hogl-Δ 1菌株中延迟，暗示Hog 1丝裂原活化蛋白激酶级联反应，这是整体应激反应的已知组分。CYC 7调控区的缺失分析表明，三个STRE元件都能够诱导应激反应。ROX 3基因的突变阻止了CYC 7 RNA在热休克和渗透胁迫过程中的积累，ROX 3 RNA水平被证明是通过一种新的调控元件由胁迫诱导的。对ROX 3温度敏感等位基因的高拷贝抑制子的选择导致了RTS 1的分离，RTS 1编码与蛋白磷酸酶2A 0的B '调节亚基具有同源性的蛋白。RTS 1的缺失引起温度和渗透压敏感性，并在所有条件下增加CYC 7 RNA的积累。该基因的过表达导致CYC 7 RNA在rox 3突变体中积累增加，但在野生型细胞中没有。
Yeast respond to a variety of stresses through a global stress response that is mediated by a number of signal transduction pathways and thecis-acting STRE DNA sequence. TheCYC7gene, encoding iso-2-cytochrome c, has been demonstrated to respond to heat shock, glucose starvation, approach-to-stationary phase, and, as we demonstrate here, to osmotic stress. This response was delayed in a thehogl-Δ1strain implicating the Hog1 mitogen-activated protein kinase cascade, a known component of the global stress response. Deletion analysis of theCYC7regulatory region suggested that three STRE elements were each capable of inducing the stress response. Mutations in theROX3gene preventedCYC7RNA accumulation during heat shock and osmotic stress.ROX3RNA levels were shown to be induced by stress through a novel regulatory element. A selection for high-copy suppressors of aROX3temperature-sensitive allele resulted in the isolation ofRTS1, encoding a protein with homology to the B′ regulatory subunit of protein phosphatase 2A0. Deletion ofRTS1caused temperature and osmotic sensitivity and increased accumulation ofCYC7RNA under all conditions. Over-expression of this gene caused increasedCYC7RNA accumulation inrox3mutants but not in wild-type cells.