Phenotypic analysis of antigen-specific T lymphocytes
Phenotypic analysis of antigen-specific T lymphocytes
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DOI：
10.1126/science.274.5284.94
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发表时间：
1996-10-04
期刊：
影响因子：
56.9
通讯作者：
Davis, MM
中科院分区：
文献类型：
--
作者：
Altman, JD;Moss, PAH;Davis, MM
免疫反应过程中抗原特异性T淋巴细胞的鉴定和表征是乏味和间接的。为了解决这个问题，对给定的T细胞群的肽 - 莫约尔组织相容性复合物（MHC）配体进行了多层次，以使可溶性肽-MHC四聚体。人类淋巴细胞抗原A2的四聚体，它们与两个不同的人免疫缺陷病毒（HIV）衍生的肽或来自甲型流感的肽与肽特异性的细胞毒性T细胞结合的基质蛋白与HIV血液中的T细胞结合到肽特异性的细胞毒性T细胞中 - 感染者。通常，四聚体结合与细胞毒性测定良好相关，该方法应在分析特异性的传染剂，肿瘤和自身抗原的T细胞中有用。
Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays, This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens.